{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:43:22Z","timestamp":1789191802632,"version":"build-2803163510"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>VirB proteins from<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>spp. constitute the type IV secretion system, a key virulence factor mediating the intracellular survival of these bacteria. Here, we assessed whether a Th1-type immune response against VirB proteins may protect mice from<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>infection and whether this response can be induced in the dog, a natural host for<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>. Splenocytes from mice immunized with VirB7 or VirB9 responded to their respective antigens with significant and specific production of gamma interferon (IFN-\u03b3), whereas interleukin-4 (IL-4) was not detected. Thirty days after an intraperitoneal challenge with live<jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>, the spleen load of bacteria was almost 1 log lower in mice immunized with VirB proteins than in unvaccinated animals. As colonization reduction seemed to correlate with a Th1-type immune response against VirB proteins, we decided to assess whether such a response could be elicited in the dog. Peripheral blood mononuclear cells (PBMCs) from dogs immunized with VirB proteins (three subcutaneous doses in QuilA adjuvant) produced significantly higher levels of IFN-\u03b3 than cells from control animals upon<jats:italic>in vitro<\/jats:italic>stimulation with VirB proteins. A skin test to assess specific delayed-type hypersensitivity was positive in 4 out of 5 dogs immunized with either VirB7 or VirB9. As both proteins are predicted to locate in the outer membrane of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>organisms, the ability of anti-VirB antibodies to mediate complement-dependent bacteriolysis of<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>was assessed<jats:italic>in vitro<\/jats:italic>. Sera from dogs immunized with either VirB7 or VirB9, but not from those receiving phosphate-buffered saline (PBS), produced significant bacteriolysis. These results suggest that VirB-specific responses that reduce organ colonization by<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>in mice can be also elicited in dogs.<\/jats:p>","DOI":"10.1128\/cvi.00653-14","type":"journal-article","created":{"date-parts":[[2014,12,25]],"date-time":"2014-12-25T05:58:17Z","timestamp":1419487097000},"page":"274-281","source":"Crossref","is-referenced-by-count":22,"title":["Immunization with Brucella VirB Proteins Reduces Organ Colonization in Mice through a Th1-Type Immune Response and Elicits a Similar Immune Response in Dogs"],"prefix":"10.1128","volume":"22","author":[{"given":"Cora N.","family":"Pollak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Magdalena","family":"Wanke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia M.","family":"Estein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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F.","family":"Staats","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,12,24]]},"references-count":41,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2015,2,25]]},"published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1128\/CVI.00653-14"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00653-14","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,24]]}}}