{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:34:41Z","timestamp":1762659281925},"reference-count":32,"publisher":"American Society for Microbiology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Diagnosis of tuberculous pleurisy remains a challenge in the clinic. In this study, we evaluated the usefulness of a previously developed<jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>antigen-specific gamma interferon enzyme-linked immunospot (ELISPOT) assay in the diagnosis of tuberculous pleurisy by testing a cohort of 352 patients with pleural effusion. We found that<jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>antigen-specific gamma interferon-producing cells were enriched four to five times in pleural fluid compared with their levels in peripheral blood from patients with tuberuclous pleurisy assayed in parallel. The sensitivity, specificity, positive predictive value, and negative predictive value of the pleural fluid mononuclear cell ELISPOT assay for the diagnosis of tuberculous pleurisy were 95.7%, 100%, 100%, and 81.0%, respectively. In comparison, the sensitivity and specificity of the ELISPOT assay using peripheral blood mononuclear cells were 78.3% and 86.3%, respectively. The sensitivity and specificity of the pleural fluid adenosine deaminase activity test were 55.5% and 86.3%, respectively. These results demonstrate that the<jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>antigen-specific ELISPOT assay performed on pleural fluid mononuclear cells provides an accurate, rapid diagnosis of tuberculous pleurisy.<\/jats:p>","DOI":"10.1128\/cvi.00680-13","type":"journal-article","created":{"date-parts":[[2014,1,4]],"date-time":"2014-01-04T04:51:44Z","timestamp":1388811104000},"page":"347-353","source":"Crossref","is-referenced-by-count":31,"title":["Gamma Interferon Immunospot Assay of Pleural Effusion Mononuclear Cells for Diagnosis of Tuberculous Pleurisy"],"prefix":"10.1128","volume":"21","author":[{"given":"Mingfeng","family":"Liao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianting","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieyun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunyi","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael W.","family":"Graner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hardy","family":"Kornfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boping","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinchun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2014,1,3]]},"reference":[{"key":"key20180919073724_B1","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1001\/archinte.163.9.1009","article-title":"The growing burden of tuberculosis: global trends and interactions with the HIV epidemic","volume":"163","author":"Corbett","year":"2003","journal-title":"Arch. Intern. Med."},{"key":"key20180919073724_B2","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/S0953-6205(03)00018-9","article-title":"Tuberculous pleural effusions","volume":"14","author":"Vald\u00e9s","year":"2003","journal-title":"Eur. J. Intern. Med."},{"key":"key20180919073724_B3","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.hrtlng.2011.04.049","article-title":"Clinical diagnostic utility of adenosine deaminase, interferon-gamma, interferon-gamma-induced protein of 10 kDa, and dipeptidyl peptidase 4 levels in tuberculous pleural effusions","volume":"41","author":"Wang","year":"2012","journal-title":"Heart Lung"},{"key":"key20180919073724_B4","first-page":"259","article-title":"Is adenosine deaminase in pleural fluid a useful marker for differentiating tuberculosis from lung cancer or mesothelioma in Japan, a country with intermediate incidence of tuberculosis?","volume":"65","author":"Ogata","year":"2011","journal-title":"Acta Med. Okayama"},{"key":"key20180919073724_B5","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1111\/j.1440-1843.2011.02053.x","article-title":"When pleural potassium exceeds 5.0 mEq\/L, high pleural adenosine deaminase levels do not necessarily indicate tuberculous pleuritis","volume":"17","author":"Kashiwabara","year":"2012","journal-title":"Respirology"},{"key":"key20180919073724_B6","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1111\/j.1469-0691.2011.03555.x","article-title":"Guidelines on interferon-gamma release assays for tuberculosis infection: concordance, discordance or confusion?","volume":"17","author":"Denkinger","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"key20180919073724_B7","first-page":"1556","article-title":"Diagnosis of latent tuberculosis infection in bacille Calmette-Guerin vaccinated subjects in China by interferon-gamma ELISpot assay","volume":"14","author":"Zhang","year":"2010","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"key20180919073724_B8","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1128\/CVI.00044-09","article-title":"Diagnosis of active tuberculosis in China using an in-house gamma interferon enzyme-linked immunospot assay","volume":"16","author":"Chen","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073724_B9","doi-asserted-by":"crossref","first-page":"S1120","DOI":"10.1093\/infdis\/jir410","article-title":"Interferon-gamma release assays for active pulmonary tuberculosis diagnosis in adults in low- and middle-income countries: systematic review and meta-analysis","volume":"204","author":"Metcalfe","year":"2011","journal-title":"J. Infect. Dis."},{"key":"key20180919073724_B10","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1111\/j.1365-2796.2008.02012.x","article-title":"Recruitment of Mycobacterium tuberculosis specific CD4+ T cells to the site of infection for diagnosis of active tuberculosis","volume":"265","author":"Nemeth","year":"2009","journal-title":"J. Intern. Med."},{"key":"key20180919073724_B11","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1111\/j.1469-0691.2008.02655.x","article-title":"Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy","volume":"15","author":"Lee","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"key20180919073724_B12","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1378\/chest.06-2063","article-title":"Diagnosis and treatment of tuberculous pleural effusion in 2006","volume":"131","author":"Gopi","year":"2007","journal-title":"Chest"},{"key":"key20180919073724_B13","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1007\/s00408-009-9165-3","article-title":"Tuberculous pleural effusion","volume":"187","author":"Porcel","year":"2009","journal-title":"Lung"},{"key":"key20180919073724_B14","doi-asserted-by":"publisher","first-page":"787","DOI":"10.5588\/ijtld.12.0892","article-title":"Can tuberculous pleural effusions be diagnosed by pleural fluid analysis alone?","volume":"17","author":"Sahn","year":"2013","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"key20180919073724_B15","doi-asserted-by":"publisher","first-page":"e30324","DOI":"10.1371\/journal.pone.0030324","article-title":"Highly accurate diagnosis of pleural tuberculosis by immunological analysis of the pleural effusion","volume":"7","author":"Sutherland","year":"2012","journal-title":"PLoS One"},{"key":"key20180919073724_B16","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1016\/j.rmed.2007.12.007","article-title":"Diagnostic accuracy of adenosine deaminase in tuberculous pleurisy: a meta-analysis","volume":"102","author":"Liang","year":"2008","journal-title":"Respir. Med."},{"key":"key20180919073724_B17","doi-asserted-by":"publisher","first-page":"143","DOI":"10.4046\/trd.2012.73.3.143","article-title":"Clinical utility of two interferon-gamma release assays on pleural fluid for the diagnosis of tuberculous pleurisy","volume":"73","author":"Kang","year":"2012","journal-title":"Tuberc. Respir. Dis. (Seoul)"},{"key":"key20180919073724_B18","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1183\/09031936.00005309","article-title":"Utility of quantitative T-cell responses versus unstimulated interferon-\u03b3 for the diagnosis of pleural tuberculosis","volume":"34","author":"Dheda","year":"2009","journal-title":"Eur. Respir. J."},{"key":"key20180919073724_B19","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1183\/09031936.00067307","article-title":"Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy","volume":"30","author":"Losi","year":"2007","journal-title":"Eur. Respir. J."},{"key":"key20180919073724_B20","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.diagmicrobio.2011.06.015","article-title":"Clinical utility of QuantiFERON-TB GOLD In-Tube and tuberculin skin test in patients with tuberculous pleural effusions","volume":"71","author":"Chung","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"key20180919073724_B21","doi-asserted-by":"publisher","first-page":"1349","DOI":"10.1086\/652142","article-title":"Rapid diagnosis of tuberculous meningitis by T cell-based assays on peripheral blood and cerebrospinal fluid mononuclear cells","volume":"50","author":"Kim","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"key20180919073724_B22","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1183\/09031936.00083707","article-title":"Rapid diagnosis of pulmonary TB by BAL enzyme-linked immunospot assay in an immunocompromised host","volume":"31","author":"Strassburg","year":"2008","journal-title":"Eur. Respir. J."},{"key":"key20180919073724_B23","first-page":"777","article-title":"Adenosine deaminase and interferon gamma measurements for the diagnosis of tuberculous pleurisy: a meta-analysis","volume":"7","author":"Greco","year":"2003","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"key20180919073724_B24","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1097\/MCP.0b013e32833a7154","article-title":"Use of pleural fluid levels of adenosine deaminase and interferon gamma in the diagnosis of tuberculous pleuritis","volume":"16","author":"Krenke","year":"2010","journal-title":"Curr. Opin. Pulm. Med."},{"key":"key20180919073724_B25","doi-asserted-by":"publisher","first-page":"752","DOI":"10.2169\/internalmedicine.31.752","article-title":"Simultaneous measurements of adenosine deaminase activity and tuberculostearic acid in pleural effusions for the diagnosis of tuberculous pleuritis","volume":"31","author":"Muranishi","year":"1992","journal-title":"Intern. Med."},{"key":"key20180919073724_B26","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1183\/09031936.96.09040747","article-title":"Adenosine deaminase (ADA) isoenzyme analysis in pleural effusions: diagnostic role, and relevance to the origin of increased ADA in tuberculous pleurisy","volume":"9","author":"Vald\u00e9s","year":"1996","journal-title":"Eur. Respir. J."},{"key":"key20180919073724_B27","first-page":"273","article-title":"Evaluation of the diagnostic value of adenosine deaminase activity in tuberculous pleuritis","volume":"33","author":"Yu","year":"2010","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"key20180919073724_B28","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1007\/s11596-007-0504-4","article-title":"Clinical value of vascular endothelial growth factor combined with interferon-gamma in diagnosing malignant pleural effusion and tuberculous pleural effusion","volume":"27","author":"Xue","year":"2007","journal-title":"J. Huazhong Univ. Sci. Technolog. Med. Sci."},{"key":"key20180919073724_B29","first-page":"435","article-title":"Clinical investigation of the diagnostic value of interferon-gamma, interleukin-12 and adenosine deaminase isoenzyme in tuberculous pleurisy","volume":"27","author":"Tian","year":"2004","journal-title":"Zhonghua Jie He He Hu Xi Za Zhi"},{"key":"key20180919073724_B30","doi-asserted-by":"publisher","first-page":"77","DOI":"10.3779\/j.issn.1009-3419.1999.02.05","article-title":"Investigation on differential diagnostic value of multi-index detection of pleural effusion","volume":"2","author":"Zhu","year":"1999","journal-title":"Zhongguo Fei Ai Za Zhi"},{"key":"key20180919073724_B31","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1159\/000066463","article-title":"Mycobacterium avium complex pleuritis","volume":"69","author":"Yanagihara","year":"2002","journal-title":"Respiration"},{"key":"key20180919073724_B32","doi-asserted-by":"publisher","first-page":"124","DOI":"10.4046\/trd.2013.74.3.124","article-title":"Mycobacterium intracellulare pleurisy identified on liquid cultures of the pleural fluid and pleural biopsy","volume":"74","author":"Lim","year":"2013","journal-title":"Tuberc. Respir. Dis. (Seoul)"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00680-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:50:15Z","timestamp":1627591815000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00680-13"}},"subtitle":[],"editor":[{"given":"V. M.","family":"Litwin","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,1,3]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,2,26]]},"published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1128\/CVI.00680-13"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00680-13","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,3]]}}}