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Chronic hepatitis C is recognized as one of the major causes of cirrhosis, hepatocellular carcinoma, and liver failure. Although new, directly acting antiviral therapies are suggested to overcome the low efficacy and adverse effects observed for the current standard of treatment, an effective vaccine would be the only way to certainly eradicate HCV infection. Recently, polyhydroxybutyrate beads produced by engineered<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>showed efficacy as a vaccine delivery system. Here, an endotoxin-free<jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>strain (ClearColi) was engineered to produce polyhydroxybutyrate beads displaying the core antigen on their surface (Beads-Core) and their immunogenicity was evaluated in BALB\/c mice. Immunization with Beads-Core induced gamma interferon (IFN-\u03b3) secretion and a functional T cell immune response against the HCV Core protein. With the aim to target broad T and B cell determinants described for HCV, Beads-Core mixed with HCV E1, E2, and NS3 recombinant proteins was also evaluated in BALB\/c mice. Remarkably, only three immunization with Beads-Core+CoE1E2NS3\/Alum (a mixture of 0.1 \u03bcg Co.120, 16.7 \u03bcg E1.340, 16.7 \u03bcg E2.680, and 10 \u03bcg NS3 adjuvanted in aluminum hydroxide [Alum]) induced a potent antibody response against E1 and E2 and a broad IFN-\u03b3 secretion and T cell response against Core and all coadministered antigens. This immunological response mediated protective immunity to viremia as assessed in a viral surrogate challenge model. Overall, it was shown that engineered biopolyester beads displaying foreign antigens are immunogenic and might present a particulate delivery system suitable for vaccination against HCV.<\/jats:p>","DOI":"10.1128\/cvi.00687-15","type":"journal-article","created":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T15:22:32Z","timestamp":1459783352000},"page":"370-378","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Protective T Cell and Antibody Immune Responses against Hepatitis C Virus Achieved Using a Biopolyester-Bead-Based Vaccine Delivery System"],"prefix":"10.1128","volume":"23","author":[{"given":"G.","family":"Mart\u00ednez-Donato","sequence":"first","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Piniella","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Olivera","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Casta\u00f1edo","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Alvarez-Lajonchere","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Due\u00f1as-Carrera","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Vaccines Department, Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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