{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T17:25:03Z","timestamp":1762017903271,"version":"3.37.3"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"SIDA fund","award":["54100020","51060029"],"award-info":[{"award-number":["54100020","51060029"]}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI100023"],"award-info":[{"award-number":["AI100023"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000061","name":"HHS | NIH | Fogarty International Center","doi-asserted-by":"publisher","award":["TW005572"],"award-info":[{"award-number":["TW005572"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP50419"],"award-info":[{"award-number":["OPP50419"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enteric fever is a systemic infection caused by typhoidal strains of\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            and is a significant cause of mortality and morbidity in many parts of the world, especially in resource-limited areas. Unfortunately, currently available diagnostic tests for enteric fever lack sensitivity and\/or specificity. No true clinically practical gold standard for diagnosing patients with enteric fever exists. Unfortunately, microbiologic culturing of blood is only 30 to 70% sensitive although 100% specific. Here, we report the development of a lateral-flow immunochromatographic dipstick assay based on the detection of\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhi (\n            <jats:italic>S<\/jats:italic>\n            . Typhi) lipopolysaccharide (LPS)-specific IgG in lymphocyte culture secretion. We tested the assay using samples from 142 clinically suspected enteric fever patients, 28 healthy individuals residing in a zone where enteric fever is endemic, and 35 patients with other febrile illnesses. In our analysis, the dipstick detected all blood culture-confirmed\n            <jats:italic>S<\/jats:italic>\n            . Typhi cases (48\/48) and 5 of 6\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Paratyphi A blood cultured-confirmed cases. The test was negative in all 35 individuals febrile with other illnesses and all 28 healthy controls from the zone of endemicity. The test was positive in 19 of 88 individuals with suspected enteric fever but with negative blood cultures. Thus, the dipstick had a sensitivity of 98% compared to blood culture results and a specificity that ranged from 78 to 100% (95% confidence interval [CI], 70 to 100%), depending on the definition of a true negative. These results suggest that this dipstick assay can be very useful for the detection of enteric fever patients especially in regions of endemicity.\n          <\/jats:p>","DOI":"10.1128\/cvi.00690-15","type":"journal-article","created":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T03:48:44Z","timestamp":1457581724000},"page":"403-409","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever"],"prefix":"10.1128","volume":"23","author":[{"given":"Iqbal Hassan","family":"Khan","sequence":"first","affiliation":[{"name":"Incepta Pharmaceuticals Ltd., Savar, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Abu","family":"Sayeed","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishat","family":"Sultana","sequence":"additional","affiliation":[{"name":"Incepta Pharmaceuticals Ltd., Savar, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamrul","family":"Islam","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakia","family":"Amin","sequence":"additional","affiliation":[{"name":"Incepta Pharmaceuticals Ltd., Savar, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Omar","family":"Faruk","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umama","family":"Khan","sequence":"additional","affiliation":[{"name":"Incepta Pharmaceuticals Ltd., Savar, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana","family":"Khanam","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward T.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002316"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.333.7558.78"},{"key":"e_1_3_2_4_2","first-page":"346","article-title":"The global burden of typhoid fever","volume":"82","author":"Crump JA","year":"2004","unstructured":"Crump JA, Luby SP, Mintz ED. 2004. The global burden of typhoid fever. Bull World Health Organ 82:346\u2013353.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/649541"},{"key":"e_1_3_2_6_2","volume-title":"Typhoid and paratyphoid fever","author":"Newton AE","year":"2013","unstructured":"Newton AE, Mintz ED. 2013. Typhoid and paratyphoid fever. In CDC health information for international travel 2013. Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/wwwnc.cdc.gov\/travel\/yellowbook\/2016\/infectious-diseases-related-to-travel\/typhoid-paratyphoid-fever."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis024"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051823"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081263"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.087627"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00311-09"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-147"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.44.3.258"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0140-6736(75)92194-7","article-title":"Relative efficacy of blood, urine, rectal swab, bone-marrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever","author":"Gilman RH","year":"1975","unstructured":"Gilman RH, Terminel M, Levine MM, Hernandez-Mendoza P, Hornick RB. 1975. Relative efficacy of blood, urine, rectal swab, bone-marrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet i:1211\u20131213.","journal-title":"Lancet"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-2-173"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.03.018"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00380-12"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.05.020"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.6.1439-1443.1993"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003619"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2010.09.041"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2011.10.016"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2010.01.017"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8305.2008.00237.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/603553"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00690-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00690-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:42:44Z","timestamp":1627591364000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00690-15"}},"subtitle":[],"editor":[{"given":"C. J.","family":"Papasian","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1128\/CVI.00690-15"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00690-15","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"type":"print","value":"1556-6811"},{"type":"electronic","value":"1556-679X"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"2015-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}