{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:03:21Z","timestamp":1743552201592},"reference-count":31,"publisher":"American Society for Microbiology","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Thirty-one bison heifers were randomly assigned to receive saline or a single vaccination with 10<jats:sup>10<\/jats:sup>CFU of<jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>strain RB51. Some vaccinated bison were randomly selected for booster vaccination with RB51 at 11 months after the initial vaccination. Mean antibody responses to RB51 were greater (<jats:italic>P<\/jats:italic>&lt; 0.05) in vaccinated bison after initial and booster vaccination than in nonvaccinated bison. The proliferative responses by peripheral blood mononuclear cells (PBMC) from the vaccinated bison were greater (<jats:italic>P<\/jats:italic>&lt; 0.05) than those in the nonvaccinated bison at 16 and 24 weeks after the initial vaccination but not after the booster vaccination. The relative gene expression of gamma interferon (IFN-\u03b3) was increased (<jats:italic>P<\/jats:italic>&lt; 0.05) in the RB51-vaccinated bison at 8, 16, and 24 weeks after the initial vaccination and at 8 weeks after the booster vaccination. The vaccinated bison had greater (<jats:italic>P<\/jats:italic>&lt; 0.05)<jats:italic>in vitro<\/jats:italic>production of IFN-\u03b3 at all sampling times, greater interleukin-1\u03b2 (IL-1\u03b2) production in various samplings after the initial and booster vaccinations, and greater IL-6 production at one sampling time after the booster vaccination. Between 170 and 180 days of gestation, the bison were intraconjunctivally challenged with approximately 1 \u00d7 10<jats:sup>7<\/jats:sup>CFU of<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>strain 2308. The incidences of abortion and infection were greater (<jats:italic>P<\/jats:italic>&lt; 0.05) in the nonvaccinated bison after experimental challenge than in the bison receiving either vaccination treatment. Booster-vaccinated, but not single-vaccinated bison, had a reduced (<jats:italic>P<\/jats:italic>&lt; 0.05) incidence of infection in fetal tissues and maternal tissues compared to that in the controls. Compared to the nonvaccinated bison, both vaccination treatments lowered the colonization (measured as the CFU\/g of tissue) of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>organisms in all tissues, except in retropharyngeal and supramammary lymph nodes. Our study suggests that RB51 booster vaccination is an effective vaccination strategy for enhancing herd immunity against brucellosis in bison.<\/jats:p>","DOI":"10.1128\/cvi.00746-14","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T06:52:45Z","timestamp":1423723965000},"page":"440-447","source":"Crossref","is-referenced-by-count":9,"title":["Immune Responses of Bison and Efficacy after Booster Vaccination with Brucella abortus Strain RB51"],"prefix":"10.1128","volume":"22","author":[{"given":"S. C.","family":"Olsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"McGill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Sacco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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