{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:42:47Z","timestamp":1792086167600,"version":"4.4.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Dense granule antigen proteins derived from<jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>(TgGRAs) are potential antigens for the development of diagnostic tools. TgGRA7 and TgGRA14 were detected in the peritoneal fluid of<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>-infected mice, suggesting that TgGRAs may be highly antigenic proteins. Here, TgGRA7 and TgGRA14 were evaluated as candidates for the development of a marker for a rapid diagnostic test. The specificity and sensitivity of purified recombinant proteins of TgGRA7 and TgGRA14 were compared in an indirect enzyme-linked immunosorbent assay (iELISA) using a series of serum samples from<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>-experimentally infected mice and using recombinant<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>major surface antigen 2 (TgSAG2) as a reference control. The iELISA with TgGRA7 showed the greatest diagnostic accuracy and could detect anti-TgGRA7 antibody in acute and chronic infections. A total of 59 field samples from pigs were also examined by the iELISAs, and the results compared with those of the latex agglutination test (LAT). Among the three recombinant antigens, TgGRA7 had the highest rates of positivity, with significant concordance (88.14) and kappa value (0.76) in comparison with the results using LAT. Furthermore, an immunochromatographic test (ICT) based on recombinant TgGRA7 was developed for rapid detection of antibodies to the infection. The ICT differentiated clearly between sera from<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>-infected mice and uninfected or<jats:named-content content-type=\"genus-species\">Neospora caninum<\/jats:named-content>-infected mice. Pig sera were examined with the ICT, and the results compared favorably with those of LAT and iELISA for TgGRA7, with kappa values of 0.66 and 0.70 to 0.79, respectively. These data suggest that the ICT based on TgGRA7 is a promising diagnostic tool for routine testing in the clinic and mass screening of samples in the field.<\/jats:p>","DOI":"10.1128\/cvi.00747-12","type":"journal-article","created":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T02:48:28Z","timestamp":1360810108000},"page":"596-601","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Development of an Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of Toxoplasma gondii Infection"],"prefix":"10.1128","volume":"20","author":[{"given":"Mohamad Alaa","family":"Terkawi","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyohko","family":"Kameyama","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazim Hamza","family":"Rasul","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuean","family":"Xuan","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshifumi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00124-7"},{"key":"e_1_3_2_3_2","first-page":"845","article-title":"Foodborne toxoplasmosis","volume":"55","author":"Jones JL Dubey JP","year":"2012","unstructured":"Jones JL DubeyJP . 2012. Foodborne toxoplasmosis. Clin. Diagn. Lab. Immunol. 55: 845\u2013 851.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.vetpar.2009.05.018","article-title":"Toxoplasmosis in pigs\u2014the last 20 years","volume":"164","author":"Dubey JP","year":"2009","unstructured":"DubeyJP . 2009. Toxoplasmosis in pigs\u2014the last 20 years. Vet. Parasitol. 164: 89\u2013 103.","journal-title":"Vet. Parasitol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2008.00345.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1086\/338827","article-title":"Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis","volume":"185","author":"Montoya JG","year":"2002","unstructured":"MontoyaJG . 2002. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J. Infect. Dis. 185(Suppl 1):S73\u2013S82.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.9.1928-1933.1990"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1144-1150.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1645\/0022-3395(2000)086[0459:UORAFD]2.0.CO;2","article-title":"Use of recombinant antigens for detection of Toxoplasma gondii infection in swine","volume":"86","author":"Gamble HR","year":"2000","unstructured":"GambleHR AndrewsCD DubeyJP WebertDW ParmleySF . 2000. Use of recombinant antigens for detection of Toxoplasma gondii infection in swine. J. Parasitol. 86: 459\u2013 462.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0304-4017(99)00004-7","article-title":"Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states","volume":"82","author":"Gamble HR","year":"1999","unstructured":"GambleHR BradyRC DubeyJP . 1999. Prevalence of Toxoplasma gondii infection in domestic pigs in the New England states. Vet. Parasitol. 82: 129\u2013 136.","journal-title":"Vet. Parasitol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.351-353.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"e421","DOI":"10.1371\/journal.pntd.0000421","article-title":"Development and evaluation of two simple, rapid immunochromatographic tests for the detection of Yersinia pestis antibodies in humans and reservoirs","volume":"3","author":"Rajerison M","year":"2009","unstructured":"RajerisonM DartevelleS RalafiarisoaLA BitamI DinhTN AndrianaivoarimananaV NatoF RahalisonL . 2009. Development and evaluation of two simple, rapid immunochromatographic tests for the detection of Yersinia pestis antibodies in humans and reservoirs. PLoS Negl. Trop. Dis. 3: e421. doi:10.1371\/journal.pntd.0000421.","journal-title":"PLoS Negl. Trop. Dis."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.diagmicrobio.2008.05.017","article-title":"Use of SAG2A recombinant Toxoplasma gondii surface antigen as a diagnostic marker for human acute toxoplasmosis: analysis of titers and avidity of IgG and IgG1 antibodies","volume":"62","author":"B\u00e9la SR","year":"2008","unstructured":"B\u00e9laSR Oliveira SilvaDA JPCunha-J\u00fanior PirovaniCP Chaves-BorgesFA Reis de CarvalhoF Carrijo de OliveiraT MineoJR . 2008. Use of SAG2A recombinant Toxoplasma gondii surface antigen as a diagnostic marker for human acute toxoplasmosis: analysis of titers and avidity of IgG and IgG1 antibodies. Diagn. Microbiol. Infect. Dis. 62:245\u2013 254.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.3347\/kjp.2009.47.S.S29","article-title":"GRA proteins of Toxoplasma gondii: maintenance of host-parasite interactions across the parasitophorous vacuolar membrane","volume":"47","author":"Nam HW","year":"2009","unstructured":"NamHW . 2009. GRA proteins of Toxoplasma gondii: maintenance of host-parasite interactions across the parasitophorous vacuolar membrane. Korean J. Parasitol. 47(Suppl):S29\u2013S37.","journal-title":"Korean J. Parasitol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0166-6851(97)00204-1","article-title":"Identification and heterologous expression of a new dense granule protein (GRA7) from Toxoplasma gondii","volume":"91","author":"Jacobs D","year":"1998","unstructured":"JacobsD DubremetzJ-F LoyensA BosmanF SamanE . 1998. Identification and heterologous expression of a new dense granule protein (GRA7) from Toxoplasma gondii. Mol. Biochem. Parasitol. 91: 237\u2013 249.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1177\/002215549804601210","article-title":"Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of Toxoplasma gondii","volume":"46","author":"Bonhomme A","year":"1998","unstructured":"BonhommeA MaineGT BeorchiaA BurletH AubertD VillenaI HuntJ ChovanL HowardL BrojanacS SheuM TynerJ PluotM PinonJM . 1998. Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of Toxoplasma gondii. J. Histochem. Cytochem. 46: 1411\u2013 1422.","journal-title":"J. Histochem. Cytochem."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00237-06"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.1.24-29.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1128\/CDLI.12.8.977-982.2005","article-title":"Seroreactivity to and avidity for recombinant antigens in toxoplasmosis","volume":"12","author":"Pfrepper KI","year":"2005","unstructured":"PfrepperKI EndersG GohlM KrczalD HlobilH WassenbergD SoutschekE . 2005. Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin. Diagn. Lab. Immunol. 12: 977\u2013 982.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00782-08"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1645\/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2","article-title":"Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats","volume":"88","author":"Huang X","year":"2002","unstructured":"HuangX XuanX KimbitaEN BatturB MiyazawaT FukumotoS MishimaM MakalaLH SuzukiH SugimotoC NagasawaH FujisakiK MikamiT IgarashiI . 2002. Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats. J. Parasitol. 88: 804\u2013 807.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0732-8893(03)00156-1","article-title":"Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis","volume":"47","author":"Nigro M","year":"2003","unstructured":"NigroM GutierrezA HofferAM ClementeM KauferF CarralL MartinV GuarneraEA AngelSO . 2003. Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis. Diagn. Microbiol. Infect. Dis. 47: 609\u2013 613.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1779-1781.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0166-6851(97)00227-2","article-title":"GRA7, an excretory 29 kDa Toxoplasma gondii dense granule antigen released by infected host cells","volume":"91","author":"Fischer H-G","year":"1998","unstructured":"FischerH-G StachelhausS SahmM MeyerHE ReichmanG . 1998. GRA7, an excretory 29 kDa Toxoplasma gondii dense granule antigen released by infected host cells. Mol. Biochem. Parasitol. 91: 251\u2013 262.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S1286-4579(02)01576-9","article-title":"Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7","volume":"4","author":"Neudeck A","year":"2002","unstructured":"NeudeckA StachelhausS NischikN StriepenB ReichmannG FischerHG . 2002. Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7. Microbes Infect. 4: 581\u2013 590.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1600-0463.2010.02629.x","article-title":"Recombinant proteins in the diagnosis of toxoplasmosis","volume":"118","author":"Kotresha D","year":"2010","unstructured":"KotreshaD NoordinR . 2010. Recombinant proteins in the diagnosis of toxoplasmosis. APMIS 118: 529\u2013 542.","journal-title":"APMIS"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.179-184.2000"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00747-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00747-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T01:35:20Z","timestamp":1714959320000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00747-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1128\/CVI.00747-12"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00747-12","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4]]},"assertion":[{"value":"2012-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}