{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:34:20Z","timestamp":1781116460580,"version":"3.54.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous studies indicated that the Lyme disease spirochete\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            expresses the RevA outer surface protein during mammalian infection. As an adhesin that promotes bacterial interaction with fibronectin, RevA appears to be a good target for preventive therapies. RevA proteins are highly conserved across all Lyme borreliae, and antibodies against RevA protein are cross-reactive among RevA proteins from diverse strains. Mice infected with\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            mounted a rapid IgM response to RevA, followed by a strong IgG response that generally remained elevated for more than 12 months, suggesting continued exposure of RevA protein to the immune system. RevA antibodies were bactericidal\n            <jats:italic>in vitro<\/jats:italic>\n            . To evaluate the RevA antigen as a potential vaccine, mice were vaccinated with recombinant RevA and challenged with\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            by inoculation with a needle or by a tick bite. Cultured tissues from all treatment groups were positive for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            . Vaccinated animals also appeared to have similar levels of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            DNA compared to nonvaccinated controls. Despite its antigenicity, surface expression, and the production of bactericidal antibodies against it, RevA does not protect against\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            infection in a mouse model. However, passive immunization with anti-RevA antibodies did prevent infection, suggesting the possible utility of RevA-based immunotherapeutics or vaccine.\n          <\/jats:p>","DOI":"10.1128\/cvi.00758-12","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T02:27:39Z","timestamp":1366252059000},"page":"892-899","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of RevA, a Fibronectin-Binding Protein of Borrelia burgdorferi, as a Potential Vaccine Candidate for Lyme Disease"],"prefix":"10.1128","volume":"20","author":[{"given":"Angela M.","family":"Floden","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Gaultney","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Brissette","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1","article-title":"Summary of notifiable diseases\u2014United States, 2009","volume":"58","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention. 2011. Summary of notifiable diseases\u2014United States, 2009. Morb. Mortal. Wkly. Rep. 58:1\u2013100.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"Stanek G","year":"2012","unstructured":"StanekGWormserGPGrayJStrleF. 2012. Lyme borreliosis. Lancet 379:461\u2013473.","journal-title":"Lancet"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0950268806007096","article-title":"The Lyme vaccine: a cautionary tale","volume":"135","author":"Nigrovic LE","year":"2007","unstructured":"NigrovicLEThompsonKM. 2007. The Lyme vaccine: a cautionary tale. Epidemiol. Infections 135:1\u20138.","journal-title":"Epidemiol. Infections"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.5.1814-1822.1993"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1016\/S0140-6736(03)14798-8","article-title":"Lyme borreliosis","volume":"362","author":"Stanek G","year":"2003","unstructured":"StanekGStrleF. 2003. Lyme borreliosis. Lancet 362:1639\u20131647.","journal-title":"Lancet"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107123450207"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.8.3698-3704.1998"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.8.3689-3697.1998"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1111\/j.1365-2958.2007.05895.x","article-title":"The role of VlsE antigenic variation in the Lyme disease spirochete: persistence through a mechanism that differs from other pathogens","volume":"65","author":"Bankhead T","year":"2007","unstructured":"BankheadTChaconasG. 2007. The role of VlsE antigenic variation in the Lyme disease spirochete: persistence through a mechanism that differs from other pathogens. Mol. Microbiol. 65:1547\u20131558.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1111\/j.1462-5822.2009.01366.x","article-title":"\u2018Nothing is permanent but change\u2019- antigenic variation in persistent bacterial pathogens","volume":"11","author":"Palmer GH","year":"2009","unstructured":"PalmerGHBankheadTLukehartSA. 2009. \u2018Nothing is permanent but change\u2019- antigenic variation in persistent bacterial pathogens. Cell. Microbiol. 11:1697\u20131705.","journal-title":"Cell. Microbiol."},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"21691","DOI":"10.1074\/jbc.M201547200","article-title":"Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi","volume":"277","author":"Eicken C","year":"2002","unstructured":"EickenCSharmaVKlabundeTLawrenzMBHardhamJMNorrisSJSacchettiniJC. 2002. Crystal structure of Lyme disease variable surface antigen VlsE of Borrelia burgdorferi. J. Biol. Chem. 277:21691\u201321696.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.tim.2007.06.003","article-title":"Hidden in plain sight: Borrelia burgdorferi and the extracellular matrix","volume":"15","author":"Cabello FC","year":"2007","unstructured":"CabelloFCGodfreyHPNewmanSA. 2007. Hidden in plain sight: Borrelia burgdorferi and the extracellular matrix. Trends Microbiol. 15:350\u2013354.","journal-title":"Trends Microbiol."},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1097\/00002281-200207000-00010","article-title":"Borrelia burgdorferi and its tropisms for adhesion molecules in the joint","volume":"14","author":"Coburn J","year":"2002","unstructured":"CoburnJMedranoMCuginiC. 2002. Borrelia burgdorferi and its tropisms for adhesion molecules in the joint. Curr. Opin. Rheumatol. 14:394\u2013398.","journal-title":"Curr. Opin. Rheumatol."},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/1529-0131(200101)44:1<151::AID-ANR19>3.0.CO;2-E","article-title":"Insights from a novel three-dimensional in vitro model of Lyme arthritis: standardized analysis of cellular and molecular interactions between Borrelia burgdorferi and synovial explants and fibroblasts","volume":"44","author":"Franz JK","year":"2001","unstructured":"FranzJKFritzeORittigMKeysserGPriemSZacherJBurmesterGRKrauseA. 2001. Insights from a novel three-dimensional in vitro model of Lyme arthritis: standardized analysis of cellular and molecular interactions between Borrelia burgdorferi and synovial explants and fibroblasts. Arthritis Rheum. 44:151\u2013162.","journal-title":"Arthritis Rheum."},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1002\/art.1780361118","article-title":"Persistence of Borrelia burgdorferi in ligamentous tissue from a patient with chronic Lyme borreliosis","volume":"36","author":"H\u00e4upl T","year":"1993","unstructured":"H\u00e4uplTHahnGRittigMKrauseASchoernerCSchonherrUKaldenJRBurmesterGR. 1993. Persistence of Borrelia burgdorferi in ligamentous tissue from a patient with chronic Lyme borreliosis. Arthritis Rheum. 36:1621\u20131626.","journal-title":"Arthritis Rheum."},{"key":"e_1_3_3_17_2","first-page":"4","volume-title":"Lyme disease and related disorders","author":"Asbrink E","year":"1988","unstructured":"AsbrinkEHovmarkA. 1988. Early and late cutaneous manifestations in Ixodes-borne borreliosis (erythema migrans borreliosis, Lyme borreliosis), p 4\u201315. In BenachJLBoslerEM (ed), Lyme disease and related disorders. New York Academy of Sciences, New York, NY."},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"S1475","DOI":"10.1093\/clinids\/11.Supplement_6.S1475","article-title":"Dermatologic manifestations of Lyme disease","volume":"11","author":"Berger BW","year":"1989","unstructured":"BergerBW. 1989. Dermatologic manifestations of Lyme disease. Rev. Infect. Dis. 11(Suppl 6):S1475\u2013S1481.","journal-title":"Rev. Infect. Dis."},{"key":"e_1_3_3_19_2","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1093\/infdis\/174.2.421","article-title":"Etiology of the acrodermatitis chronica atrophicans lesion in Lyme disease","volume":"174","author":"Ohlenbusch A","year":"1996","unstructured":"OhlenbuschAMatuschkaFRRichterDChristenHJThomssenRSpielmanAEiffertH. 1996. Etiology of the acrodermatitis chronica atrophicans lesion in Lyme disease. J. Infect. Dis. 174:421\u2013423.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00227-09"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.2174\/1568005014606062","article-title":"Adhesion mechanisms of the Lyme disease spirochete, Borrelia burgdorferi","volume":"1","author":"Coburn J","year":"2001","unstructured":"CoburnJ. 2001. Adhesion mechanisms of the Lyme disease spirochete, Borrelia burgdorferi. Curr. Drug Targets Infect. Disord. 1:171\u2013179.","journal-title":"Curr. Drug Targets Infect. Disord."},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0925-4439(98)00038-6","article-title":"Fibronectin-binding activity in Borrelia burgdorferi","volume":"1407","author":"Grab DJ","year":"1998","unstructured":"GrabDJGivensCKennedyR. 1998. Fibronectin-binding activity in Borrelia burgdorferi. Biochim. Biophys. Acta 1407:135\u2013145.","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_3_23_2","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1111\/j.1365-2958.2005.05042.x","article-title":"Inactivation of the fibronectin-binding adhesin gene bbk32 significantly attenuates the infectivity potential of Borrelia burgdorferi","volume":"59","author":"Seshu J","year":"2006","unstructured":"SeshuJEsteve-GassentMDLabandeira-ReyMKimJHTrzeciakowskiJPHookMSkareJT. 2006. Inactivation of the fibronectin-binding adhesin gene bbk32 significantly attenuates the infectivity potential of Borrelia burgdorferi. Mol. Microbiol. 59:1591\u20131601.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02035-05"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00189-06"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S1286-4579(01)01435-6","article-title":"Analysis of Borrelia burgdorferi gene expression during life cycle phases of the tick vector Ixodes scapularis","volume":"3","author":"Gilmore RD","year":"2001","unstructured":"GilmoreRDJrMbowMLStevensonB. 2001. Analysis of Borrelia burgdorferi gene expression during life cycle phases of the tick vector Ixodes scapularis. Microbes Infect. 3:799\u2013808.","journal-title":"Microbes Infect."},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00437-09"},{"key":"e_1_3_3_28_2","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1046\/j.1365-2958.2000.01698.x","article-title":"A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs of an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi","volume":"35","author":"Casjens S","year":"2000","unstructured":"CasjensSPalmerNvan VugtRHuangWMStevensonBRosaPLathigraRSuttonGPetersonJDodsonRJHaftDHickeyEGwinnMWhiteOFraserC. 2000. A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs of an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 35:490\u2013516.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6943-6952.2003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc12c01s4"},{"key":"e_1_3_3_32_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.mimet.2011.05.004","article-title":"Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi","volume":"86","author":"Bunikis I","year":"2011","unstructured":"BunikisIKutschan-BunikisSBondeMBergstromS. 2011. Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi. J. Microbiol. Methods 86:243\u2013247.","journal-title":"J. Microbiol. Methods"},{"key":"e_1_3_3_33_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1089\/vbz.2006.6.103","article-title":"A reverse transcriptase-polymerase chain reaction assay of Borrelia burgdorferi 16S rRNA for highly sensitive quantification of pathogen load in a vector","volume":"6","author":"Ornstein K","year":"2006","unstructured":"OrnsteinKBarbourAG. 2006. A reverse transcriptase-polymerase chain reaction assay of Borrelia burgdorferi 16S rRNA for highly sensitive quantification of pathogen load in a vector. Vector Borne Zoonotic Dis. 6:103\u2013112.","journal-title":"Vector Borne Zoonotic Dis."},{"key":"e_1_3_3_34_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s004300050038","article-title":"Heterogeneity in the complement-dependent bacteriolysis within the species of Borrelia burgdorferi","volume":"185","author":"Breitner-Ruddock S","year":"1997","unstructured":"Breitner-RuddockSW\u00fcrznerRSchulzeJBradeV. 1997. Heterogeneity in the complement-dependent bacteriolysis within the species of Borrelia burgdorferi. Med. Microbiol. Immunol. 185:253\u2013260.","journal-title":"Med. Microbiol. Immunol."},{"key":"e_1_3_3_35_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.ijmm.2007.09.004","article-title":"Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection","volume":"298","author":"Brissette CA","year":"2008","unstructured":"BrissetteCACooleyAEBurnsLHRileySPVermaAWoodmanMEBykowskiTStevensonB. 2008. Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection. Int. J. Med. Microbiol. 298(Suppl 1):257\u2013267.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00532-07"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1248-1251.1998"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.4.1228-1236.1997"},{"key":"e_1_3_3_39_2","doi-asserted-by":"crossref","first-page":"5286","DOI":"10.1128\/IAI.69.9.5286-5293.2001","article-title":"Borrelia burgdorferi RevA antigen is a surface-exposed outer membrane protein whose expression is regulated in response to environmental temperature and pH","volume":"69","author":"Carroll JA","year":"2001","unstructured":"CarrollJAEl-HageNMillerJCBabbKStevensonB. 2001. Borrelia burgdorferi RevA antigen is a surface-exposed outer membrane protein whose expression is regulated in response to environmental temperature and pH. Infect. Immun. 69:5286\u20135293.","journal-title":"Infect. Immun."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.980-986.1998"},{"key":"e_1_3_3_41_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1089\/153685902760173890","article-title":"Borrelia burgdorferi-specific monoclonal antibodies derived from mice primed with Lyme disease spirochete-infected Ixodes scapularis ticks","volume":"21","author":"Mbow ML","year":"2002","unstructured":"MbowMLGilmoreRDJrStevensonBGoldeWTPiesmanJJohnsonBJB. 2002. Borrelia burgdorferi-specific monoclonal antibodies derived from mice primed with Lyme disease spirochete-infected Ixodes scapularis ticks. Hybrid Hybridomics 21:179\u2013182.","journal-title":"Hybrid Hybridomics"},{"key":"e_1_3_3_42_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6976.2010.00243.x","article-title":"Fibronectin: a multidomain host adhesin targeted by bacterial fibronectin-binding proteins","volume":"35","author":"Henderson B","year":"2011","unstructured":"HendersonBNairSPallasJWilliamsMA. 2011. Fibronectin: a multidomain host adhesin targeted by bacterial fibronectin-binding proteins. FEMS Microbiol. Rev. 35:147\u2013200.","journal-title":"FEMS Microbiol. Rev."},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc12c03s00"},{"key":"e_1_3_3_45_2","doi-asserted-by":"crossref","first-page":"5612","DOI":"10.4049\/jimmunol.1103735","article-title":"Delays and diversions mark the development of B cell responses to Borrelia burgdorferi infection","volume":"188","author":"Hastey CJ","year":"2012","unstructured":"HasteyCJElsnerRABartholdSWBaumgarthN. 2012. Delays and diversions mark the development of B cell responses to Borrelia burgdorferi infection. J. Immunol. 188:5612\u20135622.","journal-title":"J. Immunol."},{"key":"e_1_3_3_46_2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0171-2985(11)80504-8","article-title":"Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies","volume":"181","author":"Kramer MD","year":"1990","unstructured":"KramerMDSchaibleUEWallichRMoterSEPetzoldtDSimonMM. 1990. Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Immunobiology 181:357\u2013366.","journal-title":"Immunobiology"},{"key":"e_1_3_3_47_2","doi-asserted-by":"crossref","first-page":"S46","DOI":"10.1086\/338463","article-title":"Approaches toward the directed design of a vaccine against Borrelia burgdorferi","volume":"185","author":"Luft BJ","year":"2002","unstructured":"LuftBJDunnJJLawsonCL. 2002. Approaches toward the directed design of a vaccine against Borrelia burgdorferi. J. Infect. Dis. 185(Suppl 1):S46\u2013S51.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_3_48_2","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1093\/infdis\/157.4.842","article-title":"Experimental Lyme arthritis in rats infected with Borrelia burgdorferi","volume":"157","author":"Barthold SW","year":"1988","unstructured":"BartholdSWMoodyKDTerwilligerGADurayPHJacobyROSteereAC. 1988. Experimental Lyme arthritis in rats infected with Borrelia burgdorferi. J. Infect. Dis. 157:842\u2013846.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_3_49_2","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1056\/NEJM198303313081301","article-title":"The spirochetal etiology of Lyme disease","volume":"308","author":"Steere AC","year":"1983","unstructured":"SteereACGrodzickiRLKornblattANCraftJEBarbourAGBurgdorferWSchmidGPJohnsonEMalawistaSE. 1983. The spirochetal etiology of Lyme disease. N. Engl. J. Med. 308:733\u2013740.","journal-title":"N. Engl. J. Med."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00758-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00758-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:49:26Z","timestamp":1627591766000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00758-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1128\/CVI.00758-12"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00758-12","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6]]},"assertion":[{"value":"2012-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}