{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:46:23Z","timestamp":1759009583610},"reference-count":28,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Pertussis remains an important public health problem in many countries despite extensive immunization. Cultures and real-time PCR (RT-PCR) assays are the recommended pertussis diagnostic tests, but they lack sensitivity at the later stage of the disease. This study introduces the IgG anti-pertussis toxin enzyme-linked immunosorbent assay (PT ELISA) in our routine diagnosis to improve disease burden estimation. Serum samples and nasopharyngeal swabs (<jats:italic>n<\/jats:italic>= 503) were collected at the same time from patients presenting with cough illness suspected of being pertussis and tested by the PT ELISA and culture and\/or RT-PCR, respectively. Patients were separated into three age groups: group 1, &lt;1 year (<jats:italic>n<\/jats:italic>= 260; mean age, 3 months), group 2, 1 to 6 years (<jats:italic>n<\/jats:italic>= 81; mean age, 3 years), and group 3, \u22657 years (<jats:italic>n<\/jats:italic>= 162; mean age, 26 years). The times (means) from cough onset to specimen collection were 16, 24, and 26 days, respectively. In group 1, 83 (82.2%) of 101 positive cases were positive for pertussis by culture\/RT-PCR, while 40 (39.6%) tested positive by PT ELISA. In group 2, 6 (19.4%) of 31 positive cases were culture\/RT-PCR positive, and 29 (93.6%) were seropositive. In group 3, 13 (13.8%) of 94 positive cases were positive by culture\/RT-PCR and 91 (96.8%) were positive by serology. Culture\/RT-PCR detected more cases of pertussis in infants (<jats:italic>P<\/jats:italic>&lt; 0.0001), whereas the PT ELISA detected more cases in adolescents and adults (<jats:italic>P<\/jats:italic>&lt; 0.0001). The timing between cough onset and specimen collection or recent vaccination may have partially affected our results. Serology is a suitable, cost-effective, and complementary pertussis diagnostic tool, especially among older children, adolescents, and adults during the later disease phase.<\/jats:p>","DOI":"10.1128\/cvi.00760-13","type":"journal-article","created":{"date-parts":[[2014,3,6]],"date-time":"2014-03-06T05:12:56Z","timestamp":1394082776000},"page":"636-640","source":"Crossref","is-referenced-by-count":11,"title":["Serodiagnosis as Adjunct Assay for Pertussis Infection in S\u00e3o Paulo, Brazil"],"prefix":"10.1128","volume":"21","author":[{"given":"Lourdes R. A.","family":"Vaz-de-Lima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monte D.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia C.","family":"Pawloski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Leite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen C. P.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyro A.","family":"de Brito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia M. I.","family":"Vaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano Moura","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielly P.","family":"Alvarenga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana F.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Telma R. M. P.","family":"Carvalhanas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa M. 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Pertussis (whooping cough)","author":"Pan American Health Organization\/World Health Organization","year":"2012"},{"key":"key20180919073730_B2","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1017\/S095026881200091X","article-title":"The pertussis epidemic: informing strategies for prevention of severe disease","volume":"141","author":"Clarke","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"key20180919073730_B3","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s00431-007-0548-2","article-title":"What is new in pertussis?","volume":"167","author":"Bamberger","year":"2008","journal-title":"Eur. J. Pediatr."},{"key":"key20180919073730_B4","unstructured":"Minist\u00e9rio Da Sa\u00fade. 2014. Minist\u00e9rio da Sa\u00fade alerta sobre a situa\u00e7\u00e3o epidemiol\u00f3gica da coqueluche no Brasil. http:\/\/portalsaude.saude.gov.br\/index.php\/profissional-e-gestor\/vigilancia\/noticias-vigilancia\/7569-."},{"key":"key20180919073730_B5","unstructured":"Minist\u00e9rio Da Sa\u00fade. 2012. Vacina adsorvida difteria, t\u00e9tano e pertussis (acelular)\u2014dTpa\u2014para vacina\u00e7\u00e3o de gestantes. http:\/\/u.saude.gov.br\/images\/pdf\/2014\/janeiro\/28\/VacinadTPa-Gestantes-final.pdf."},{"key":"key20180919073730_B6","first-page":"20","article-title":"Doen\u00e7as de transmiss\u00e3o respirat\u00f3ria","volume":"27","author":"Secretaria de Estado da Sa\u00fade de S\u00e3o Paulo","year":"2006","journal-title":"Bol. Epidemiol. Paulista"},{"key":"key20180919073730_B7","first-page":"4","article-title":"Current status and perspective of worldwide disease burden of pertussis","volume":"12","author":"Meng","year":"2006","journal-title":"Chin J. 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