{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T18:34:51Z","timestamp":1781116491650,"version":"3.54.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            <jats:italic>bba64<\/jats:italic>\n            gene product is a surface-localized lipoprotein synthesized within mammalian and tick hosts and is involved in vector transmission of disease. These properties suggest that BBA64 may be a vaccine candidate against Lyme borreliosis. In this study, protective immunity against\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            challenge was assessed in mice immunized with the BBA64 protein. Mice developed a high-titer antibody response following immunization with soluble recombinant BBA64 but were not protected when challenged by needle inoculation of culture-grown spirochetes. Likewise, mice passively immunized with an anti-BBA64 monoclonal antibody were not protected against needle-inoculated organisms. BBA64-immunized mice were subjected to\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            challenge by the natural route of a tick bite, but these trials did not demonstrate significant protective immunity in either outbred or inbred strains of mice. Lipidated recombinant BBA64 produced in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            was assessed for possible improved elicitation of a protective immune response. Although inoculation with this antigen produced a high-titer antibody response, the lipidated BBA64 also was unsuccessful in protecting mice from\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            challenge by tick bites. Anti-BBA64 antibodies raised in rats eradicated the organisms, as evidenced by\n            <jats:italic>in vitro<\/jats:italic>\n            borreliacidal assays, thus demonstrating the potential for BBA64 to be effective as a protective immunogen. However, passive immunization with the same monospecific rat anti-BBA64 polyclonal serum failed to provide protection against tick bite-administered challenge. These results reveal the challenges faced in not only identifying\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            proteins with potential protective capability but also in producing recombinant antigens conducive to preventive therapies against Lyme borreliosis.\n          <\/jats:p>","DOI":"10.1128\/cvi.00824-13","type":"journal-article","created":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T03:09:28Z","timestamp":1391656168000},"page":"526-533","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of the Borrelia burgdorferi BBA64 Protein as a Protective Immunogen in Mice"],"prefix":"10.1128","volume":"21","author":[{"given":"Kevin S.","family":"Brandt","sequence":"first","affiliation":[{"name":"Bacterial Diseases Branch, Division of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toni G.","family":"Patton","sequence":"additional","affiliation":[{"name":"Bacterial Diseases Branch, Division of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S.","family":"Allard","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J.","family":"Caimano","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Pediatrics, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D.","family":"Radolf","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Pediatrics, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Immunology, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut, USA"},{"name":"Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Gilmore","sequence":"additional","affiliation":[{"name":"Bacterial Diseases Branch, Division of Vector Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/508667"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.5419-5432.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2012.00980.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.121"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/651172"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq116"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.271"},{"key":"e_1_3_2_9_2","first-page":"209","article-title":"Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant","volume":"339","author":"Steere AC","year":"1998","unstructured":"SteereACSikandVKMeuriceFParentiDLFikrigESchoenRTNowakowskiJSchmidCHLaukampSBuscarinoCKrauseDS. 1998. 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