{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T02:33:31Z","timestamp":1787884411533,"version":"build-2784847793"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The second-generation MVista\n            <jats:named-content content-type=\"genus-species\">Blastomyces<\/jats:named-content>\n            antigen enzyme immunoassay was not quantitative; therefore, specimens obtained previously were tested in the same assay as new specimens to assess the change in antigen levels. Furthermore, the sensitivity in serum had not been fully evaluated. The purpose of this study was to evaluate a quantitative\n            <jats:named-content content-type=\"genus-species\">Blastomyces<\/jats:named-content>\n            antigen assay and detection of antigen in serum. Calibrators containing known concentrations of\n            <jats:named-content content-type=\"genus-species\">Blastomyces<\/jats:named-content>\n            galactomannan were used to quantify antigen in urine and serum from patients with proven blastomycosis and from controls. Paired current and previously obtained urine specimens were tested to determine if quantification eliminated the need for concurrent testing to assess change in antigen. Pretreatment of serum with EDTA at 104\u00b0C was evaluated to determine if dissociation of immune complexes improved detection of antigenemia. Antigenuria was detected in 89.9% of patients with culture- or histopathology-proven blastomycosis. Specificity was 99.0% in patients with nonfungal infections and healthy subjects, but cross-reactions occurred in 95.6% of patients with histoplasmosis. Change in antigen level categorized as increase, no change, or decrease based on antigen units determined in the same assay agreed closely with the category of change in ng\/ml determined from different assays. Pretreatment increased the sensitivity of detection of antigenemia from 35.7% to 57.1%. Quantification eliminated the need for concurrent testing of current and previously obtained specimens for assessment of changes in antigen concentration. Pretreatment increased the sensitivity for detection of antigenemia. Differentiation of histoplasmosis and blastomycosis is not possible by antigen detection.\n          <\/jats:p>","DOI":"10.1128\/cvi.05248-11","type":"journal-article","created":{"date-parts":[[2011,11,24]],"date-time":"2011-11-24T06:11:27Z","timestamp":1322115087000},"page":"53-56","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["Blastomyces dermatitidis Antigen Detection by Quantitative Enzyme Immunoassay"],"prefix":"10.1128","volume":"19","author":[{"given":"Patricia","family":"Connolly","sequence":"first","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chadi A.","family":"Hage","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine and Roudebush Veterans' Administration Medical Center, Pulmonary Critical Care Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Ryan","family":"Bariola","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Bensadoun","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine, University of Kentucky, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Rodgers","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Robert W.","family":"Bradsher","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Joseph","family":"Wheat","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"119","article-title":"Approximate is better than \u201cexact\u201d for interval estimation of binomial proportions","volume":"52","author":"Agresti A","year":"2009","unstructured":"AgrestiA CoullBA. 2009. Approximate is better than \u201cexact\u201d for interval estimation of binomial proportions. Am. Stat. 52: 119\u2013216.","journal-title":"Am. Stat."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1097\/md.0b013e3180679130","article-title":"Systemic histoplasmosis: a 15-year retrospective institutional review of 111 patients","volume":"86","author":"Assi MA","year":"2007","unstructured":"AssiMA SandidMS BaddourLM RobertsGD WalkerRC. 2007. Systemic histoplasmosis: a 15-year retrospective institutional review of 111 patients. Medicine (Baltimore) 86: 162\u2013169.","journal-title":"Medicine (Baltimore)"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.diagmicrobio.2010.09.015","article-title":"Detection of Blastomyces dermatitidis antigen in patients with newly diagnosed blastomycosis","volume":"69","author":"Bariola JR","year":"2011","unstructured":"BariolaJR. 2011. Detection of Blastomyces dermatitidis antigen in patients with newly diagnosed blastomycosis. Diagn. Microbiol. Infect. Dis. 69: 187\u2013191.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1378\/chest.10-0627","article-title":"Blastomycosis in Indiana: digging up more cases","volume":"138","author":"Carlos WG","year":"2010","unstructured":"CarlosWG. 2010. Blastomycosis in Indiana: digging up more cases. Chest 138: 1377\u20131382.","journal-title":"Chest"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00071-07"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4873-4875.2004"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u20133062.2011.00658.x"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00389-10"},{"key":"e_1_3_1_10_2","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1093\/cid\/cir435","article-title":"A multicenter evaluation of tests for diagnosis of histoplasmosis","volume":"53","author":"Hage CA","year":"2011","unstructured":"HageCA. 2011. A multicenter evaluation of tests for diagnosis of histoplasmosis. Clin. Infect. Dis. 53: 448\u2013454.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1111\/j.1939-1676.2008.0107.x","article-title":"Antigen and antibody testing for the diagnosis of blastomycosis in dogs","volume":"22","author":"Spector D","year":"2008","unstructured":"SpectorD. 2008. Antigen and antibody testing for the diagnosis of blastomycosis in dogs. J. Vet. Intern. Med. 22: 839\u2013843.","journal-title":"J. Vet. Intern. Med."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00409-08"},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1086\/648421","article-title":"Diagnosis of acute pulmonary histoplasmosis by antigen detection","volume":"49","author":"Swartzentruber S","year":"2009","unstructured":"SwartzentruberS. 2009. Diagnosis of acute pulmonary histoplasmosis by antigen detection. Clin. Infect. Dis. 49: 1878\u20131882.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_1_14_2","doi-asserted-by":"crossref","first-page":"89059","DOI":"10.1155\/2007\/89059","article-title":"Blastomyces antigen detection for monitoring progression of blastomycosis in a pregnant adolescent","volume":"2007","author":"Tarr M","year":"2007","unstructured":"TarrM. 2007. Blastomyces antigen detection for monitoring progression of blastomycosis in a pregnant adolescent. Infect. Dis. Obstet. Gynecol. 2007: 89059.","journal-title":"Infect. Dis. Obstet. Gynecol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.05248-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.05248-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:46:11Z","timestamp":1627591571000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.05248-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1128\/CVI.05248-11"],"URL":"https:\/\/doi.org\/10.1128\/cvi.05248-11","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"2011-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}