{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:35:01Z","timestamp":1777368901552,"version":"3.51.4"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Congenital transmission of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            occurs mainly when a mother acquires the infection for the first time during pregnancy. It was recently shown that although early treatment of the primary infection during pregnancy has little or no impact on the fetomaternal transmission rate, it does reduce the incidence of sequelae in infected infants. Seroconversion is defined by the appearance of IgG. Commercial reagents continue to vary considerably in detecting low concentrations of antibodies, as during early seroconversion. We compared two routinely used immunoassays (IA) (Platelia and Elecsys Toxo IgG) and an indirect immunofluorescence assay (IIF) with a qualitative test based on immunoblot analysis (Toxo II IgG) (IB) to assess their abilities to diagnose seroconversion at its earliest stages. This prospective study was carried out between January and November 2010. It included 39 pregnant women with monthly follow-up who seroconverted during pregnancy. On first sera that were IgM positive but IgG negative (or equivocal) as detected by IA, IB diagnosed seroconversion twice as often as IIF (26\/39 [66.7%] versus 13\/39 [33.3%];\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001; \u03c7\n            <jats:sup>2<\/jats:sup>\n            test). Serum samples were retaken 2 to 5 weeks later for the other 13 cases (IgG negative by IB on first serum). Seroconversion was demonstrated as follows: IB for 5 cases where IA remained negative or equivocal, IB and IIF for 5 cases where IA remained negative or equivocal, IA for 2 cases, and no method for 1 case (a third sample was necessary). In summary, IB permitted toxoplasmosis seroconversion diagnosis before other means in 92.3% of cases (36\/39) and thus earlier therapeutic intervention.\n          <\/jats:p>","DOI":"10.1128\/cvi.05303-11","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T11:31:15Z","timestamp":1314876675000},"page":"1908-1912","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Utility of Immunoblotting for Early Diagnosis of Toxoplasmosis Seroconversion in Pregnant Women"],"prefix":"10.1128","volume":"18","author":[{"given":"C.","family":"Jost","sequence":"first","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Touafek","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Fekkar","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"},{"name":"Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S945, Paris F-75013, France"},{"name":"INSERM, U945, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Courtin","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Mazier","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"},{"name":"Universit\u00e9 Pierre et Marie Curie-Paris 6, UMR S945, Paris F-75013, France"},{"name":"INSERM, U945, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Paris","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Service de Parasitologie-Mycologie, Paris F-75013, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","volume-title":"Toxoplasmose: \u00e9tat des connaissances et \u00e9valuation du risque li\u00e9 \u00e0 l'alimentation\u2014 rapport du groupe de travail \u201cToxoplasma gondii.\u201d","author":"Agence Fran\u00e7aise de S\u00e9curit\u00e9 Sanitaire des Aliments.","year":"2006","unstructured":"Agence Fran\u00e7aise de S\u00e9curit\u00e9 Sanitaire des Aliments. 2006. Toxoplasmose: \u00e9tat des connaissances et \u00e9valuation du risque li\u00e9 \u00e0 l'alimentation\u2014 rapport du groupe de travail \u201cToxoplasma gondii.\u201d Agence Fran\u00e7aise de S\u00e9curit\u00e9 Sanitaire des Aliments, Maisons-Alfort, France."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1016\/S0140-6736(98)08220-8","article-title":"Mother to child transmission of toxoplasmosis: risk estimates for clinical counseling","volume":"353","author":"Dunn D.","year":"1999","unstructured":"DunnD. 1999. Mother to child transmission of toxoplasmosis: risk estimates for clinical counseling. Lancet 353:1829\u20131833.","journal-title":"Lancet"},{"key":"e_1_3_1_4_2","first-page":"125","article-title":"Toxoplasma gondii serology in pregnant woman: characteristics and pitfalls","volume":"67","author":"Flori P.","year":"2009","unstructured":"FloriP. CheneG. VarletM. N. SungR. T. 2009. Toxoplasma gondii serology in pregnant woman: characteristics and pitfalls. Ann. Biol. Clin. (Paris) 67:125\u2013133.","journal-title":"Ann. Biol. Clin. (Paris)"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/S0002-9378(99)70224-3","article-title":"Treatment of toxoplasmosis during pregnancy: a multicenter study of impact on fetal transmission and children's sequelae at age 1 year","volume":"180","author":"Foulon W.","year":"1999","unstructured":"FoulonW. 1999. Treatment of toxoplasmosis during pregnancy: a multicenter study of impact on fetal transmission and children's sequelae at age 1 year. Am. J. Obstet. Gynecol. 180:410\u2013415.","journal-title":"Am. J. Obstet. Gynecol."},{"key":"e_1_3_1_6_2","volume-title":"Congress of the French Society of Parasitology-Parasites in Mother and Child-2005","author":"Franck J.","year":"2005","unstructured":"FranckJ. DerocleG. Sa\u00efzA. J. DumonH. 2005. Evaluation of the kit LDBIO-TOXO II IgG for the confirmation of IgG results obtained by toxoplasmosis serology screening tests, poster. Congress of the French Society of Parasitology-Parasites in Mother and Child-2005, 15-16th December, Paris, France."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-08"},{"key":"e_1_3_1_8_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1590\/S0074-02762009000200026","article-title":"Treatment for congenital toxoplasmosis: finding out what works. Mem","volume":"104","author":"Gilbert R","year":"2009","unstructured":"GilbertR 2009. Treatment for congenital toxoplasmosis: finding out what works. Mem. Inst. Oswaldo Cruz 104:305\u2013311.","journal-title":"Inst. 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World Health Organ."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/j.1469-0691.2006.01444.x","article-title":"Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis","volume":"12","author":"Sensini A","year":"2006","unstructured":"SensiniA 2006. Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis. Clin. Microbiol. Infect. 12:504\u2013512.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_1_19_2","first-page":"267","article-title":"Performance of Elecsys toxo IgG and IgM immunoassays","volume":"55","author":"Van Helden J","year":"2009","unstructured":"Van HeldenJ 2009. Performance of Elecsys toxo IgG and IgM immunoassays. Clin. Lab. 55:267\u2013273.","journal-title":"Clin. Lab."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.05303-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.05303-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:44:30Z","timestamp":1627591470000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.05303-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":18,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1128\/CVI.05303-11"],"URL":"https:\/\/doi.org\/10.1128\/cvi.05303-11","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]},"assertion":[{"value":"2011-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-08-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}