{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:31:09Z","timestamp":1789731069946,"version":"4.0.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Helicobacter pylori<\/jats:named-content>\n            infection is prevalent worldwide and results in chronic gastritis, which may lead to gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer. We have previously reported that oral immunization with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium smegmatis<\/jats:named-content>\n            expressing the\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            outer membrane protein 26-kilodalton (Omp26) antigen affords therapeutic protection against\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            infection in mice. In the present study, we investigated the prophylactic effects of this vaccine candidate on\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            challenge in mice. We found that oral immunization with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            Omp26 significantly reduced\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            colonization in the stomach compared to inoculation with wild-type\n            <jats:named-content content-type=\"genus-species\">M. smegmatis<\/jats:named-content>\n            in control mice. Six of the recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            -immunized mice (60%) were completely protected from\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            infection. The severity of\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            -associated chronic gastritis assessed histologically was significantly milder in mice vaccinated with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            than in control animals. Mice immunized with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            showed enhanced antigen-specific lymphocyte proliferation and antibody responses. Moreover, immunization with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            resulted in an increased expression of interleukin-2 and gamma interferon in the stomach and spleen, as determined by reverse transcription-PCR analysis. Our results collectively suggest that vaccination with recombinant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            Omp26 confers prophylactic protection against\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            infection. The inhibition of\n            <jats:named-content content-type=\"genus-species\">H. pylori<\/jats:named-content>\n            colonization is associated with the induction of antigen-specific humoral and cell-mediated immune responses.\n          <\/jats:p>","DOI":"10.1128\/cvi.05306-11","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T02:48:13Z","timestamp":1315450093000},"page":"1957-1961","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Oral Immunization with Recombinant Mycobacterium smegmatis Expressing the Outer Membrane Protein 26-Kilodalton Antigen Confers Prophylactic Protection against Helicobacter pylori Infection"],"prefix":"10.1128","volume":"18","author":[{"given":"Lin","family":"L\u00fc","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-qing","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Hematology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pi-long","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-xiu","family":"Xiang","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Epigenetics Laboratory, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-chuan","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"6977","DOI":"10.4049\/jimmunol.169.12.6977","article-title":"Protection against Helicobacter pylori infection following immunization is IL-12-dependent and mediated by Th1 cells","volume":"169","author":"Akhiani A. 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