{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:10:34Z","timestamp":1793211034361,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As a consequence of continued spillover of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n            into cattle from wildlife reservoirs and increased globalization of cattle trade with associated transmission risks, new approaches such as vaccination and novel testing algorithms are seriously being considered by regulatory agencies for the control of bovine tuberculosis. Serologic tests offer opportunities for identification of\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            -infected animals not afforded by current diagnostic techniques. The present study describes assay development and field assessment of a new commercial enzyme-linked immunosorbent assay (ELISA) that detects antibody to\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            antigens MPB83 and MPB70 in infected cattle. Pertinent findings include the following: specific antibody responses were detected at \u223c90 to 100 days after experimental\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            challenge, minimal cross-reactive responses were elicited by infection\/sensitization with nontuberculous\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            spp., and the apparent sensitivity and specificity of the ELISA with naturally infected cattle were 63% and 98%, respectively, with sensitivity improving as disease severity increased. The ELISA also detected infected animals missed by the routine tuberculin skin test, and antibody was detectable in bulk tank milk samples from\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            -infected dairy herds. A high-throughput ELISA could be adapted as a movement, border, or slaughter surveillance test, as well as a supplemental test to tuberculin skin testing.\n          <\/jats:p>","DOI":"10.1128\/cvi.05343-11","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T09:32:17Z","timestamp":1316079137000},"page":"1882-1888","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle"],"prefix":"10.1128","volume":"18","author":[{"given":"W. R.","family":"Waters","sequence":"first","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. M.","family":"Buddle","sequence":"additional","affiliation":[{"name":"AgResearch, Hopkirk Research Institute, Palmerston North, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. M.","family":"Vordermeier","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency, Addlestone, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Gormley","sequence":"additional","affiliation":[{"name":"School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. V.","family":"Palmer","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. C.","family":"Thacker","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Bannantine","sequence":"additional","affiliation":[{"name":"National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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Protection of cattle from bovine tuberculosis by vaccination with BCG by the respiratory or subcutaneous route, but not by vaccination with killed Mycobacterium vaccae. Res. Vet. Sci. 59:10\u201316.","journal-title":"Res. Vet. Sci"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1574-6968.1991.tb04380.x","article-title":"Identification of an insertion sequence IS1081, in Mycobacterium bovis","volume":"67","author":"Collins D. M.","year":"1991","unstructured":"CollinsD. M. StephensD. M.. 1991. Identification of an insertion sequence IS1081, in Mycobacterium bovis. FEMS Microbiol. Lett. 67:11\u201315.","journal-title":"FEMS Microbiol. Lett"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/00480169.2003.36343","article-title":"The re-emergence of Mycobacterium bovis infection in brushtail possums (Trichosurus vulpecula) after localised possum eradication","volume":"51","author":"Corner L. 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