{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T14:34:29Z","timestamp":1759847669931},"reference-count":24,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Serodiagnosis of amoebiasis remains the preferred method for diagnosis of amoebic liver abscess (ALA). However, the commercially available kits are problematic in areas of endemicity due to the persistently high background antibody titers. Human serum samples (\n            <jats:italic>n<\/jats:italic>\n            = 38) from patients with ALA who live in areas of endemicity were collected from Hospital Universiti Sains Malaysia during the period of 2008 to 2010. Western blots using excretory-secretory antigen (ESA) collected from axenically grown\n            <jats:named-content content-type=\"genus-species\">Entamoeba histolytica<\/jats:named-content>\n            were probed with the above serum samples. Seven antigenic proteins of ESA with various reactivities were identified, i.e., 152 kDa, 131 kDa, 123 kDa, 110 kDa, 100 kDa, 82 kDa, and 76 kDa. However, only the 152-kDa and 110-kDa proteins showed sensitivities above 80% in the Western blot analysis. All the antigenic proteins showed undetectable cross-reactivity when probed with healthy human serum samples (\n            <jats:italic>n<\/jats:italic>\n            = 30) and serum samples from other infections (\n            <jats:italic>n<\/jats:italic>\n            = 33). From the matrix-assisted laser desorption ionization\u2013two-stage time of flight (MALDI-TOF\/TOF) analysis, the proteins were identified as heavy subunits of\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            lectin and\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            pyruvate phosphate dikinase, respectively. Use of the\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            lectin for diagnosis of ALA has been well reported by researchers and is being used in commercialized kits. However, this is the first report on the potential use of pyruvate phosphate dikinase for diagnosis of ALA; thus, this molecule merits further evaluation on its diagnostic value using a larger panel of serum samples.\n          <\/jats:p>","DOI":"10.1128\/cvi.05356-11","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T09:32:17Z","timestamp":1316079137000},"page":"1913-1917","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Analysis of Entamoeba histolytica Excretory-Secretory Antigen and Identification of a New Potential Diagnostic Marker"],"prefix":"10.1128","volume":"18","author":[{"given":"Weng Kin","family":"Wong","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi Ning","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Health Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurulhasanah","family":"Othman","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boon Huat","family":"Lim","sequence":"additional","affiliation":[{"name":"School of Health Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeehaida","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Olivos Garcia","sequence":"additional","affiliation":[{"name":"Departmento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, 04510 Mexico D.F., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmah","family":"Noordin","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s002689900573","article-title":"Amebic liver abscess: changing trends over 20 years","volume":"23","author":"Akgun Y.","year":"1999","unstructured":"AkgunY. 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Amoebic liver abscess. S. S. Publishers Bombay India. http:\/\/www.bhj.org\/books\/liver\/s7c02.htm."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1089\/fpd.2009.0427","article-title":"Application of real-time polymerase chain reaction in detection of Entamoeba histolytica in pus aspirates of amoebic liver abscess patients","volume":"7","author":"Othman N.","year":"2010","unstructured":"OthmanN. 2010. Application of real-time polymerase chain reaction in detection of Entamoeba histolytica in pus aspirates of amoebic liver abscess patients. Foodborne Pathog. Dis. 7:637\u2013641.","journal-title":"Foodborne Pathog. Dis."},{"key":"e_1_3_2_11_2","first-page":"152","article-title":"Comparative evaluation of somatic and excretory-secretory antigens of Entamoeba histolytica in serodiagnosis of human amoebiasis by ELISA","volume":"104","author":"Pal S.","year":"1996","unstructured":"PalS. 1996. Comparative evaluation of somatic and excretory-secretory antigens of Entamoeba histolytica in serodiagnosis of human amoebiasis by ELISA. Indian J. Med. Res. 104:152\u2013156.","journal-title":"Indian J. Med. Res."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.exppara.2006.11.005","article-title":"Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview","volume":"116","author":"Paul J.","year":"2007","unstructured":"PaulJ. SrivastavaS. BhattacharyaS. 2007. Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview. Exp. Parasitol. 116:35\u201343.","journal-title":"Exp. 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Role of excretory-secretory products of Entamoeba histolytica in human amebiasis. Arch. Med. Res. 31:S226\u2013S228.","journal-title":"Arch. Med. Res."},{"key":"e_1_3_2_17_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s10822-007-9130-2","article-title":"Molecular modeling on pyruvate phosphate dikinase of Entamoeba histolytica and in silico virtual screening for novel inhibitors","volume":"22","author":"Stephen P.","year":"2008","unstructured":"StephenP. 2008. Molecular modeling on pyruvate phosphate dikinase of Entamoeba histolytica and in silico virtual screening for novel inhibitors. J. Comput. Aided Mol. Des. 22:647\u2013660.","journal-title":"J. Comput. Aided Mol. 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Comparison of protein-free defined media, and effect of l-cysteine and ascorbic acid supplementation on viability of axenic Entamoeba histolytica. Parasitol. Res. 108:425\u2013430.","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_22_2","first-page":"13","article-title":"WHO\/PAHO\/UNESCO report","volume":"18","author":"World Health Organization.","year":"1997","unstructured":"World Health Organization. 1997. WHO\/PAHO\/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico 28\u201329 January, 1997. Epidemiol. Bull. 18:13\u201314.","journal-title":"A consultation with experts on amoebiasis. Mexico City, Mexico 28\u201329 January, 1997. Epidemiol. Bull."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/S1471-4922(02)02289-4","article-title":"How protozoan parasites evade the immune response","volume":"18","author":"Zambrano-Villa S.","year":"2002","unstructured":"Zambrano-VillaS. 2002. 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