{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:57:47Z","timestamp":1761541067829},"reference-count":74,"publisher":"American Society for Microbiology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A cDNA encoding a 16.5-kDa protein termed FhTP16.5 was identified by immunoscreening of a cDNA library from<jats:named-content content-type=\"genus-species\">Fasciola hepatica<\/jats:named-content>adult flukes using pooled sera from rabbits infected with<jats:named-content content-type=\"genus-species\">F. hepatica<\/jats:named-content>for 4 weeks. Quantitative reverse transcriptase PCR (qPCR) analysis revealed that FhTP16.5 is not expressed in unembryonated eggs. It is poorly expressed in miracidia and highly expressed at the juvenile and adult stages; however, significant differences were found between the expression levels of FhTP16.5 in juveniles versus adult flukes. Recombinant FhTP16.5 was expressed at high levels in<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>, purified by affinity chromatography, and used to raise anti-FhTP16.5 polyclonal antibodies in rabbits. Immunoblot analysis using the anti-FhTP16.5 IgG antibody identified FhTP16.5 in crude and tegumental extracts and in excretory-secretory products of<jats:named-content content-type=\"genus-species\">F. hepatica<\/jats:named-content>. The protein was not detected in crude extracts of<jats:named-content content-type=\"genus-species\">Schistosoma mansoni<\/jats:named-content>or<jats:named-content content-type=\"genus-species\">Schistosoma<\/jats:named-content><jats:named-content content-type=\"genus-species\">japonicum<\/jats:named-content>. Antibodies to FhTP16.5 were detected in the sera of rabbits at 3 to 12 weeks of<jats:named-content content-type=\"genus-species\">F. hepatica<\/jats:named-content>infection as well as in the sera of humans with chronic fascioliasis; these findings suggest that FhTP16.5 could be a good antigen for serodiagnosis of fascioliasis. Immunohistochemistry demonstrated that FhTP16.5 localizes to the surface of the tegument of various developmental stages and in parenchymal tissues of the adult fluke. Such specific localization makes FhTP16.5 an attractive target for immunoprophylaxis or chemotherapy.<\/jats:p>","DOI":"10.1128\/cvi.05501-11","type":"journal-article","created":{"date-parts":[[2012,1,26]],"date-time":"2012-01-26T06:13:29Z","timestamp":1327558409000},"page":"325-333","source":"Crossref","is-referenced-by-count":22,"title":["Biochemical Characterization and Differential Expression of a 16.5-Kilodalton Tegument-Associated Antigen from the Liver Fluke Fasciola hepatica"],"prefix":"10.1128","volume":"19","author":[{"given":"Jos\u00e9 F.","family":"Gaudier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Cab\u00e1n-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Osuna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Espino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2012,1,25]]},"reference":[{"key":"key20180919073556_B1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0304-4017(00)00263-6","article-title":"Vaccination of mice against Schistosoma bovis with a recombinant fatty acid binding protein from Fasciola hepatica.","volume":"91","author":"Abane","year":"2000","journal-title":"Vet. 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