{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T16:54:02Z","timestamp":1775840042373,"version":"3.50.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Screening of an expression library of<jats:named-content content-type=\"genus-species\">Leptospira interrogans<\/jats:named-content>with eye fluids from uveitic horses resulted in identification of a novel protein, LruC. LruC is located in the inner leaflet of the leptospiral outer membrane, and an<jats:italic>lruC<\/jats:italic>gene was detected in all tested pathogenic<jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>strains. LruC-specific antibody levels were significantly higher in eye fluids and sera of uveitic horses than healthy horses. These findings suggest that LruC may play a role in equine leptospiral uveitis.<\/jats:p>","DOI":"10.1128\/cvi.05524-11","type":"journal-article","created":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T06:00:24Z","timestamp":1326348024000},"page":"452-456","source":"Crossref","is-referenced-by-count":27,"title":["Antibodies to a Novel Leptospiral Protein, LruC, in the Eye Fluids and Sera of Horses with Leptospira-Associated Uveitis"],"prefix":"10.1128","volume":"19","author":[{"given":"Ashutosh","family":"Verma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Matsunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Artiushin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marija","family":"Pinne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk J.","family":"Houwers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Haake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Stevenson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Timoney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2012,1,11]]},"reference":[{"key":"key20180919073559_B1","first-page":"27","article-title":"Equine recurrent uveitis: current concepts in diagnosis and treatment","volume":"12","author":"Abrams","year":"1990","journal-title":"Equine Pract."},{"key":"key20180919073559_B2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0378-1119(98)00329-1","article-title":"Molecular analysis of the dnaK locus of Leptospira interrogans serovar Copenhageni","volume":"216","author":"Ballard","year":"1998","journal-title":"Gene"},{"key":"key20180919073559_B3","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","article-title":"Improved prediction of signal peptides: SignalP 3.0","volume":"340","author":"Bendtsen","year":"2004","journal-title":"J. Mol. Biol."},{"key":"key20180919073559_B4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/BF01739964","article-title":"Caldesmon, acidic amino acids and molecular weight determinations","volume":"10","author":"Bryan","year":"1989","journal-title":"J. Muscle Res. Cell Motil."},{"key":"key20180919073559_B5","doi-asserted-by":"crossref","first-page":"14560","DOI":"10.1073\/pnas.0603979103","article-title":"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential","volume":"103","author":"Bulach","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919073559_B6","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1086\/515273","article-title":"Identification of Leptospira species in the pathogenesis of uveitis and determination of clinical ocular characteristics in south India","volume":"177","author":"Chu","year":"1998","journal-title":"J. Infect. Dis."},{"key":"key20180919073559_B7","first-page":"2","article-title":"Equine recurrent uveitis","volume":"2","author":"Cook","year":"1983","journal-title":"Equine Vet. J."},{"key":"key20180919073559_B8","doi-asserted-by":"crossref","first-page":"4853","DOI":"10.1128\/IAI.73.8.4853-4863.2005","article-title":"Surfaceome of Leptospira spp","volume":"73","author":"Cullen","year":"2005","journal-title":"Infect. Immun."},{"key":"key20180919073559_B9","volume-title":"Leptospira and leptospirosis","author":"Faine","year":"1999","edition":"2nd ed"},{"key":"key20180919073559_B10","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0165-2427(99)00082-3","article-title":"Characterization of T-lymphocytes in the anterior uvea of eyes with chronic equine recurrent uveitis","volume":"77","author":"Gilger","year":"1999","journal-title":"Vet. Immunol. Immunopathol."},{"key":"key20180919073559_B11","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1128\/IAI.59.3.1131-1140.1991","article-title":"Changes in the surface of Leptospira interrogans serovar grippotyphosa during in vitro cultivation","volume":"59","author":"Haake","year":"1991","journal-title":"Infect. Immun."},{"key":"key20180919073559_B12","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1128\/IAI.68.4.2276-2285.2000","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection","volume":"68","author":"Haake","year":"2000","journal-title":"Infect. Immun."},{"key":"key20180919073559_B13","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1099\/00221287-146-7-1491","article-title":"Spirochaetal lipoproteins and pathogenesis","volume":"146","author":"Haake","year":"2000","journal-title":"Microbiology"},{"key":"key20180919073559_B14","doi-asserted-by":"crossref","first-page":"4936","DOI":"10.1128\/IAI.70.9.4936-4945.2002","article-title":"Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins","volume":"70","author":"Haake","year":"2002","journal-title":"Infect. Immun."},{"key":"key20180919073559_B15","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.3109\/02713688509003348","article-title":"Studies on equine recurrent uveitis. II. The role of infection with Leptospira interrogans serovar Pomona","volume":"4","author":"Halliwell","year":"1985","journal-title":"Curr. Eye Res."},{"key":"key20180919073559_B16","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1439-0450.2004.00740.x","article-title":"Classification of leptospira from the eyes of horses suffering from recurrent uveitis","volume":"51","author":"Hartskeerl","year":"2004","journal-title":"J. Vet. Med. B"},{"key":"key20180919073559_B17","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0966-842X(01)01984-9","article-title":"The ALS gene family of Candida albicans","volume":"9","author":"Hoyer","year":"2001","journal-title":"Trends Microbiol."},{"key":"key20180919073559_B18","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1110\/ps.0303703","article-title":"Prediction of lipoprotein signal peptides in Gram-negative bacteria","volume":"12","author":"Juncker","year":"2003","journal-title":"Protein Sci."},{"key":"key20180919073559_B19","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1076\/ocii.6.4.239.4026","article-title":"Retinal immunopathology in horses with uveitis","volume":"6","author":"Kalsow","year":"1998","journal-title":"Ocul. Immunol. Inflamm."},{"key":"key20180919073559_B20","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1073\/pnas.82.4.958","article-title":"Anomalous interaction of the acetylcholine receptor protein with the nonionic detergent Triton X-114","volume":"82","author":"Maher","year":"1985","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919073559_B21","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1046\/j.1365-2958.2003.03619.x","article-title":"Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily","volume":"49","author":"Matsunaga","year":"2003","journal-title":"Mol. Microbiol."},{"key":"key20180919073559_B22","doi-asserted-by":"crossref","first-page":"3777","DOI":"10.1099\/mic.0.29162-0","article-title":"LipL46 is a novel surface-exposed lipoprotein expressed during leptospiral dissemination in the mammalian host","volume":"152","author":"Matsunaga","year":"2006","journal-title":"Microbiology"},{"key":"key20180919073559_B23","article-title":"Uveitis in horses. Mod. Vet. Pract. 351-357","author":"Miller","year":"1987"},{"key":"key20180919073559_B24","first-page":"436","article-title":"Equine leptospirosis","volume":"155","author":"Morter","year":"1969","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"key20180919073559_B25","doi-asserted-by":"crossref","first-page":"7616","DOI":"10.1128\/IAI.69.12.7616-7624.2001","article-title":"Molecular characterization of thermoinduced immunogenic proteins Qlp42 and Hsp15 of Leptospira interrogans","volume":"69","author":"Nally","year":"2001","journal-title":"Infect. Immun."},{"key":"key20180919073559_B26","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1128\/JB.186.7.2164-2172.2004","article-title":"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis","volume":"186","author":"Nascimento","year":"2004","journal-title":"J. Bacteriol."},{"key":"key20180919073559_B27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0165-2427(85)90048-0","article-title":"Experimental detection of an antigenic relationship between Leptospira and equine cornea","volume":"10","author":"Parma","year":"1985","journal-title":"Vet. Immunol. Immunopathol."},{"key":"key20180919073559_B28","doi-asserted-by":"crossref","first-page":"e6071","DOI":"10.1371\/journal.pone.0006071","article-title":"A comprehensive approach to identification of surface-exposed, outer membrane-spanning proteins of Leptospira interrogans","volume":"4","author":"Pinne","year":"2009","journal-title":"PLoS One"},{"key":"key20180919073559_B29","first-page":"2805","article-title":"Immuno-fluorescence assay of leptospiral surface-exposed proteins","volume":"2011","author":"Pinne","year":"2011","journal-title":"J. Vis. Exp."},{"key":"key20180919073559_B30","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1038\/nature01597","article-title":"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing","volume":"422","author":"Ren","year":"2003","journal-title":"Nature"},{"key":"key20180919073559_B31","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1099\/mic.0.28317-0","article-title":"Lipoprotein computational prediction in spirochaetal genomes","volume":"152","author":"Setubal","year":"2006","journal-title":"Microbiology"},{"key":"key20180919073559_B32","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1128\/IAI.64.6.2322-2330.1996","article-title":"Molecular cloning and sequence analysis of the gene encoding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species","volume":"64","author":"Shang","year":"1996","journal-title":"Infect. 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