{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:04:22Z","timestamp":1787652262765,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Leptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species of<jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of the<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>heat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5\u00d7 the 50% lethal dose (LD<jats:sub>50<\/jats:sub>) of<jats:named-content content-type=\"genus-species\">Leptospira interrogans<\/jats:named-content>, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (<jats:italic>P<\/jats:italic>&lt; 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.<\/jats:p>","DOI":"10.1128\/cvi.05720-11","type":"journal-article","created":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T05:39:35Z","timestamp":1330580375000},"page":"740-745","source":"Crossref","is-referenced-by-count":47,"title":["Protection against Lethal Leptospirosis after Vaccination with LipL32 Coupled or Coadministered with the B Subunit of Escherichia coli Heat-Labile Enterotoxin"],"prefix":"10.1128","volume":"19","author":[{"given":"Andr\u00e9 A.","family":"Grassmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel R.","family":"F\u00e9lix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Ximendes dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta G.","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amilton C. 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