{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:34:11Z","timestamp":1790127251971,"version":"4.0.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An assay capable of distinguishing between the immune response generated by recent exposure to rubella virus and the immune response existing as a result of past exposure or immunization is required for the diagnosis of primary rubella virus infection, especially in pregnant women. Avidity assays, which are based on the premise that chaotropic agents can be used to selectively dissociate the low-avidity antibodies generated early in the course of infection, have become routinely used in an effort to accomplish this. We have thoroughly investigated the immunological basis of an avidity assay using a viral lysate-based assay and an enzyme-linked immunosorbent assay (ELISA) based on a peptide analogue of the putative immunodominant region of the E1 glycoprotein (E1\n            <jats:sup>208-239<\/jats:sup>\n            ). The relative affinities of the antibodies directed against E1\n            <jats:sup>208-239<\/jats:sup>\n            were measured by surface plasmon resonance and were found to correlate well with the avidity index calculated from the ELISA results. We found that the immune response generated during primary rubella virus infection consists of an initial low-affinity peak of immunoglobulin M (IgM) reactivity followed by transient peaks of low-avidity IgG3 and IgA reactivity. The predominant response is an IgG1 response which increases in concentration and affinity progressively over the course of infection. Incubation with the chaotropic agent used in the avidity assay abolished the detection of the early low-affinity peaks of IgM, IgA, and IgG3 reactivity while leaving the high-affinity IgG1 response relatively unaffected. The present study supported the premise that avidity assays based on appropriate antigens can be useful to confirm primary rubella virus infection.\n          <\/jats:p>","DOI":"10.1128\/cvi.13.3.380-386.2006","type":"journal-article","created":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T18:48:00Z","timestamp":1141757280000},"page":"380-386","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Humoral Immune Response to Primary Rubella Virus Infection"],"prefix":"10.1128","volume":"13","author":[{"given":"Kim M.","family":"Wilson","sequence":"first","affiliation":[{"name":"National Serology Reference Laboratory, St. Vincent's Institute of Medical Research, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlie","family":"Di Camillo","sequence":"additional","affiliation":[{"name":"National Serology Reference Laboratory, St. Vincent's Institute of Medical Research, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Doughty","sequence":"additional","affiliation":[{"name":"National Serology Reference Laboratory, St. Vincent's Institute of Medical Research, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M.","family":"Dax","sequence":"additional","affiliation":[{"name":"National Serology Reference Laboratory, St. Vincent's Institute of Medical Research, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1136\/bmj.325.7356.147","volume":"325","year":"2002","unstructured":"Best, J. M., S. O'Shea, G. Tipples, N. Davies, S. M. Al Khusaiby, A. Krause, L. M. Hesketh, L. Jin, and G. Enders. 2002. Interpretation of rubella serology in pregnancy\u2014pitfalls and problems. BMJ325:147-148.","journal-title":"BMJ"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1089\/aid.1997.13.1007","volume":"13","year":"1997","unstructured":"Binley, J. M., H. Arshad, T. R. Fouts, and J. P. Moore. 1997. An investigation of the high-avidity antibody response to glycoprotein 120 of human immunodeficiency virus type 1. AIDS Res. Hum. Retrovir.13:1007-1015.","journal-title":"AIDS Res. Hum. Retrovir."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1093\/infdis\/172.3.897","volume":"172","year":"1995","unstructured":"Chargelegue, D., C. M. Stanley, C. M. O'Toole, B. T. Colvin, and M. W. Steward. 1995. 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