{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:55:36Z","timestamp":1789757736716,"version":"4.0.1"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The triggering receptor expressed on myeloid cells (TREM-1) is a recently identified receptor expressed on neutrophils and monocytes. Activation of the receptor induces neutrophils to release the enzyme myeloperoxidase and inflammatory cytokines such as interleukin-8. TREM-1 has an alternatively spliced variant that lacks the transmembrane region, resulting in the receptor being secreted in a soluble form (sTREM-1). Soluble TREM-1 has been detected in plasma during experimental and clinical sepsis and has been advocated as a diagnostic marker of infection for pneumonia and as a prognostic marker for patients with septic shock. We studied TREM-1 surface expression, using flow cytometry, and simultaneously measured sTREM-1 concentrations in culture supernatants of lipopolysaccharide (LPS)-stimulated neutrophils. TREM-1 surface expression was constitutive and was not upregulated upon LPS stimulation. However, sTREM-1 release from neutrophils was significantly upregulated by LPS stimulation (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001), an effect that was abrogated by cycloheximide. Soluble TREM-1 is therefore secreted by human neutrophils in response to LPS challenge in a process involving de novo protein synthesis that is not accompanied by an upregulation of the TREM-1 receptor on the surfaces of the cells.\n          <\/jats:p>","DOI":"10.1128\/cvi.13.4.492-495.2006","type":"journal-article","created":{"date-parts":[[2006,4,7]],"date-time":"2006-04-07T15:13:26Z","timestamp":1144422806000},"page":"492-495","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Production of Soluble Triggering Receptor Expressed on Myeloid Cells by Lipopolysaccharide-Stimulated Human Neutrophils Involves De Novo Protein Synthesis"],"prefix":"10.1128","volume":"13","author":[{"given":"Amr M.","family":"Mahdy","sequence":"first","affiliation":[{"name":"Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damon A.","family":"Lowes","sequence":"additional","affiliation":[{"name":"Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen F.","family":"Galley","sequence":"additional","affiliation":[{"name":"Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Bruce","sequence":"additional","affiliation":[{"name":"Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel R.","family":"Webster","sequence":"additional","affiliation":[{"name":"Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"S109","DOI":"10.1097\/00003246-200107001-00035","volume":"29","year":"2001","unstructured":"Angus, D. C., and R. S. Wax. 2001. Epidemiology of sepsis: an update. Crit. Care Med.29:S109-S116.","journal-title":"Crit. Care Med."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.4049\/jimmunol.170.7.3812","volume":"170","year":"2003","unstructured":"Bleharski, J. R., V. Kiessler, C. Buonsanti, P. A. Sieling, S. Stenger, M. Colonna, and R. L. Modlin. 2003. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response. J. Immunol.170:3812-3818.","journal-title":"J. Immunol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"4991","DOI":"10.4049\/jimmunol.164.10.4991","volume":"164","year":"2000","unstructured":"Bouchon, A., J. Dietrich, and M. Colonna. 2000. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J. Immunol.164:4991-4995.","journal-title":"J. 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