{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:06:05Z","timestamp":1761487565225},"reference-count":21,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A simple, rapid, field-adapted indirect enzyme-linked immunoassay (FldELISA) for the detection of antibodies to\n            <jats:italic>Brucella abortus<\/jats:italic>\n            in whole blood and serum has been developed. This assay detects antibodies to\n            <jats:italic>B. abortus<\/jats:italic>\n            in approximately 15 min or less. Over a 3-month period, this assay has consistently identified immunized and nonimmunized animals, while the percent coefficient of variation for each immunized animal has been less than 20%. As with any indirect enzyme-linked immunoassay, quality control can be established and maintained. Using defined positive and negative sera, the sensitivity and specificity of the FldELISA was 100% and 94.2%, respectively. As a model, this test can be readily extended to other disease applications that use lipopolysaccharide or other stable antigens for the detection of antibodies, such as those to\n            <jats:italic>Salmonella<\/jats:italic>\n            spp.,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , or\n            <jats:italic>Yersinia<\/jats:italic>\n            spp.\n          <\/jats:p>","DOI":"10.1128\/cvi.13.4.501-506.2006","type":"journal-article","created":{"date-parts":[[2006,4,7]],"date-time":"2006-04-07T15:13:26Z","timestamp":1144422806000},"page":"501-506","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Rapid, Field-Adapted Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies in Bovine Whole Blood and Serum to\n            <i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"D.","family":"Gall","sequence":"first","affiliation":[{"name":"Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Yu","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Smith","sequence":"additional","affiliation":[{"name":"Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada K2H 8P9"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"349","volume":"56","year":"1984","unstructured":"Angus, R. 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