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It is important to understand how the signaling pathways that regulate these factors involved in virulence are orchestrated. Protein phosphatases are central to numerous signal transduction pathways. Here, we characterize the<jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>protein phosphatase 2A SitA, the<jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>Sit4p homologue. The<jats:italic>sitA<\/jats:italic>gene is not an essential gene, and we were able to construct an<jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>null mutant. The \u0394<jats:italic>sitA<\/jats:italic>strain had decreased MpkA phosphorylation levels, was more sensitive to cell wall-damaging agents, had increased \u03b2-(1,3)-glucan and chitin, was impaired in biofilm formation, and had decreased protein kinase C activity. The \u0394<jats:italic>sitA<\/jats:italic>strain is more sensitive to several metals and ions, such as MnCl<jats:sub>2<\/jats:sub>, CaCl<jats:sub>2<\/jats:sub>, and LiCl, but it is more resistant to ZnSO<jats:sub>4<\/jats:sub>. The \u0394<jats:italic>sitA<\/jats:italic>strain was avirulent in a murine model of invasive pulmonary aspergillosis and induces an augmented tumor necrosis factor alpha (TNF-\u03b1) response in mouse macrophages. These results stress the importance of<jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>SitA as a possible modulator of PkcA\/MpkA activity and its involvement in the cell wall integrity pathway.<\/jats:p>","DOI":"10.1128\/ec.00008-15","type":"journal-article","created":{"date-parts":[[2015,4,25]],"date-time":"2015-04-25T00:19:56Z","timestamp":1429921196000},"page":"728-744","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["The Aspergillus fumigatus sitA Phosphatase Homologue Is Important for Adhesion, Cell Wall Integrity, Biofilm Formation, and Virulence"],"prefix":"10.1128","volume":"14","author":[{"given":"Vin\u00edcius Leite Pedro","family":"Bom","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, 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