{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:56:46Z","timestamp":1791824206932,"version":"4.3.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            is commonly responsible for lethal fungal infections among immunosuppressed individuals.\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            forms biofilm communities that are of increasing biomedical interest due to the association of biofilms with chronic infections and their increased resistance to antifungal agents and host immune factors. Understanding the composition of microbial biofilms and the extracellular matrix is important to understanding function and, ultimately, to developing strategies to inhibit biofilm formation. We implemented a solid-state nuclear magnetic resonance (NMR) approach to define compositional parameters of the\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            extracellular matrix (ECM) when biofilms are formed in RPMI 1640 nutrient medium. Whole biofilm and isolated matrix networks were also characterized by electron microscopy, and matrix proteins were identified through protein gel analysis. The\n            <jats:sup>13<\/jats:sup>\n            C NMR results defined and quantified the carbon contributions in the insoluble ECM, including carbonyls, aromatic carbons, polysaccharide carbons (anomeric and nonanomerics), aliphatics, etc. Additional\n            <jats:sup>15<\/jats:sup>\n            N and\n            <jats:sup>31<\/jats:sup>\n            P NMR spectra permitted more specific annotation of the carbon pools according to C-N and C-P couplings. Together these data show that the\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            ECM produced under these growth conditions contains approximately 40% protein, 43% polysaccharide, 3% aromatic-containing components, and up to 14% lipid. These fundamental chemical parameters are needed to consider the relationships between composition and function in the\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            ECM and will enable future comparisons with other organisms and with\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            grown under alternate conditions.\n          <\/jats:p>","DOI":"10.1128\/ec.00050-15","type":"journal-article","created":{"date-parts":[[2015,7,11]],"date-time":"2015-07-11T02:25:45Z","timestamp":1436581545000},"page":"1064-1072","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["Analysis of the Aspergillus fumigatus Biofilm Extracellular Matrix by Solid-State Nuclear Magnetic Resonance Spectroscopy"],"prefix":"10.1128","volume":"14","author":[{"given":"Courtney","family":"Reichhardt","sequence":"first","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A. G.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, California, USA"},{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia-Marie","family":"Joubert","sequence":"additional","affiliation":[{"name":"Cell Sciences Imaging Facility, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl V.","family":"Clemons","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, California, USA"},{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, California, USA"},{"name":"California Institute for Medical Research, San Jose, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette","family":"Cegelski","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Stanford University, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02104-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802247694"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-199705000-00059"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.502190"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00895.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2009.00482.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-014-9766-0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2013.819592"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47247-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-53"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-1-3"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201002823"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0202326"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja8039426"},{"key":"e_1_3_3_16_2","first-page":"743","volume-title":"Solid-state NMR studies of biopolymers","author":"Toke O","year":"2010","unstructured":"Toke O, Cegelski L. 2010. 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