{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:50:26Z","timestamp":1781279426931,"version":"3.54.1"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Candidiasis now represents the fourth most frequent nosocomial infection both in the United States and worldwide.<jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>is an increasingly common threat to human health as a consequence of AIDS, steroid therapy, organ and tissue transplantation, cancer therapy, broad-spectrum antibiotics, and other immune defects. The pathogenic potential of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>is intimately related to certain key processes, including biofilm formation and filamentation. Ddr48p is a damage response protein that is significantly upregulated during both biofilm formation and filamentation, but its actual function is unknown. Previous studies have indicated that this protein may be essential in<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>but not<jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>. Here we examined the function of Ddr48p and investigated the role of this protein in biofilm formation and filamentation. We demonstrated that this protein is not essential in<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>and appears to be dispensable for filamentation. However,<jats:italic>DDR48<\/jats:italic>is required for the flocculation response stimulated by 3-aminotriazole-induced amino acid starvation. Furthermore, we examined the response of this deletion strain to a wide variety of environmental stressors and antifungal compounds. We observed several mild sensitivity or resistance phenotypes and also found that Ddr48p contributes to the DNA damage response of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>. The results of this study reveal that the role of this highly expressed protein goes beyond a general stress response and impinges on a key facet of pathogenesis, namely, the ability to sense and respond to changes in the host environment.<\/jats:p>","DOI":"10.1128\/ec.00107-12","type":"journal-article","created":{"date-parts":[[2012,4,21]],"date-time":"2012-04-21T06:48:54Z","timestamp":1334990934000},"page":"718-724","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Investigating the Function of Ddr48p in Candida albicans"],"prefix":"10.1128","volume":"11","author":[{"given":"I. A.","family":"Cleary","sequence":"first","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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P.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Grand Valley State University, Allendale, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfj161"},{"key":"e_1_3_2_3_2","first-page":"86S","article-title":"Secular trends in nosocomial primary bloodstream infections in the United States, 1980-1989","volume":"91","author":"Banerjee SN","year":"1991","unstructured":"BanerjeeSN. 1991. Secular trends in nosocomial primary bloodstream infections in the United States, 1980-1989. National Nosocomial Infections Surveillance System. Am. J. Med. 91:86S\u201389S.","journal-title":"National Nosocomial Infections Surveillance System. Am. J. 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