{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T01:00:22Z","timestamp":1785632422285,"version":"3.56.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Entamoeba histolytica<\/jats:named-content>\n            , the protist that causes amebic dysentery and liver abscess, has a truncated Asn-linked glycan (\n            <jats:italic>N<\/jats:italic>\n            -glycan) precursor composed of seven sugars (Man\n            <jats:sub>5<\/jats:sub>\n            GlcNAc\n            <jats:sub>2<\/jats:sub>\n            ). Here, we show that glycoproteins with unmodified\n            <jats:italic>N<\/jats:italic>\n            -glycans are aggregated and capped on the surface of\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            trophozoites by the antiretroviral lectin cyanovirin-N and then replenished from large intracellular pools. Cyanovirin-N cocaps the Gal\/GalNAc adherence lectin, as well as glycoproteins containing\n            <jats:italic>O<\/jats:italic>\n            -phosphodiester-linked glycans recognized by an anti-proteophosphoglycan monoclonal antibody. Cyanovirin-N inhibits phagocytosis by\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            trophozoites of mucin-coated beads, a surrogate assay for amebic virulence. For technical reasons, we used the plant lectin concanavalin A rather than cyanovirin-N to enrich secreted and membrane proteins for mass spectrometric identification.\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            glycoproteins with occupied\n            <jats:italic>N<\/jats:italic>\n            -glycan sites include Gal\/GalNAc lectins, proteases, and 17 previously hypothetical proteins. The latter glycoproteins, as well as 50 previously hypothetical proteins enriched by concanavalin A, may be vaccine targets as they are abundant and unique. In summary, the antiretroviral lectin cyanovirin-N binds to well-known and novel targets on the surface of\n            <jats:named-content content-type=\"genus-species\">E. histolytica<\/jats:named-content>\n            that are rapidly replenished from large intracellular pools.\n          <\/jats:p>","DOI":"10.1128\/ec.00166-10","type":"journal-article","created":{"date-parts":[[2010,9,18]],"date-time":"2010-09-18T02:45:13Z","timestamp":1284777913000},"page":"1661-1668","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["The Antiretroviral Lectin Cyanovirin-N Targets Well-Known and Novel Targets on the Surface of Entamoeba histolytica Trophozoites"],"prefix":"10.1128","volume":"9","author":[{"given":"Andrea","family":"Carpentieri","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Ratner","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudip K.","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulagna","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Guy","family":"Bushkin","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jike","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lubrano","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Steffen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Boston University Medical School, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E.","family":"Costello","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Resource, Department of Biochemistry, Boston University Medical Center, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"O'Keefe","sequence":"additional","affiliation":[{"name":"Molecular Targets Development Program, Center for Cancer Research, NCI-Frederick, Frederick, Maryland 21702"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillips W.","family":"Robbins","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Samuelson","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.04.010"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4358-4367.1995"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp941"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704862104"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099005259"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.3.501-509.2003"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.12.007"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.4.5.657"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(07)65002-7"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905818106"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000415"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050058i"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq020"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg537"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1994.1104"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049937f"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.10.4243-4249.1997"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(96)10033-0"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022710"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.011303.073752"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2004.8.357"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr700859x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4315"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01188"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-006-0675-1"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-38"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00493-06"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03291"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp184"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800725200"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.8.3248"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.9.1557"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.96"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3577"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/12.1.3"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.10.001"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901506106"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.56.012302.160959"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90177-L"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qai.0000187446.76579.d3"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00268-08"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.2.292-297.1985"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409460102"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.14.7306-7321.2002"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200600182X"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.4976"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90250-N"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.034926"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00361-0"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01455-07"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001442"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.110701.153949"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.5.836-848.2006"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj091"},{"key":"e_1_3_3_57_2","first-page":"553","article-title":"Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry","volume":"112","author":"Yates J. R.","year":"1999","unstructured":"Yates J. R. III Carmack E. Hays L. Link A. J. Eng J. K. . 1999. Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry. Methods Mol. Biol.112:553\u2013569.","journal-title":"Methods Mol. Biol."}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00166-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00166-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:43:51Z","timestamp":1645613031000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.00166-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":56,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1128\/EC.00166-10"],"URL":"https:\/\/doi.org\/10.1128\/ec.00166-10","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]},"assertion":[{"value":"2010-07-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}