{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:25:30Z","timestamp":1791192330183,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In yeast cells such as those of\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            , expression of ATP-binding cassette (ABC) transporter proteins has been found to be increased and correlates with a concomitant elevation in azole drug resistance. In this study, we investigated the roles of two\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            proteins that share high sequence similarity with\n            <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n            Pdr5, an ABC transporter protein that is commonly overproduced in azole-resistant isolates in this yeast. The two\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            genes encoding the ABC transporters sharing the highest sequence similarity to\n            <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n            Pdr5 are called\n            <jats:italic>abcA<\/jats:italic>\n            and\n            <jats:italic>abcB<\/jats:italic>\n            here. We constructed deletion alleles of these two different ABC transporter-encoding genes in three different strains of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Loss of\n            <jats:italic>abcB<\/jats:italic>\n            invariably elicited increased azole susceptibility, while\n            <jats:italic>abcA<\/jats:italic>\n            disruption alleles had variable phenotypes. Specific antibodies were raised to both AbcA and AbcB proteins. These antisera allowed detection of AbcB in wild-type cells, while AbcA could be visualized only when overproduced from the\n            <jats:italic>hspA<\/jats:italic>\n            promoter in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Overproduction of AbcA also yielded increased azole resistance. Green fluorescent protein fusions were used to provide evidence that both AbcA and AbcB are localized to the plasma membrane in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . Promoter fusions to firefly luciferase suggested that expression of both ABC transporter-encoding genes is inducible by azole challenge. Virulence assays implicated AbcB as a possible factor required for normal pathogenesis. This work provides important new insights into the physiological roles of ABC transporters in this major fungal pathogen.\n          <\/jats:p>","DOI":"10.1128\/ec.00171-13","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:21:49Z","timestamp":1381540909000},"page":"1619-1628","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Contributions of Aspergillus fumigatus ATP-Binding Cassette Transporter Proteins to Drug Resistance and Virulence"],"prefix":"10.1128","volume":"12","author":[{"given":"Sanjoy","family":"Paul","sequence":"first","affiliation":[{"name":"Departments of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Diekema","sequence":"additional","affiliation":[{"name":"Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"},{"name":"Pathology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Scott","family":"Moye-Rowley","sequence":"additional","affiliation":[{"name":"Departments of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"},{"name":"Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq279"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)31588-1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42155-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41760-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00310491"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00254-07"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.12.012"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.49.31543"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.12.6875"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.25.14712"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.4.2098"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00352101"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.11.2378"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.7.1695"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2009.09.002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050219"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1087-1845(02)00016-6"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.5.1719-1726.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-006-0073-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00167-13"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt075"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-177"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2003.12.003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.1.207-211.2006"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2006.06.005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(08)60245-X"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.4.1798-1802.2005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.6.1495"},{"key":"e_1_3_2_30_2","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989","unstructured":"SambrookJFritschEFManiatisT. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"2"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00174-12"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/122.1.19"},{"key":"e_1_3_2_33_2","volume-title":"Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard M38-A2","author":"CLSI","year":"2008","unstructured":"CLSI. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard M38-A2, 2nd ed. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.4.2998"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5061868.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-06-0519"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.21.12322"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.3.1147"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2013.06.004"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.1.212-215.2006"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00414-06"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01092-06"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1639-1652.2004"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3773-3781.2004"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.11.5879"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00171-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00171-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T15:17:15Z","timestamp":1645629435000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.00171-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":44,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1128\/EC.00171-13"],"URL":"https:\/\/doi.org\/10.1128\/ec.00171-13","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"2013-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}