{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:47:51Z","timestamp":1790621271077,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genome of the basidiomycete pathogenic yeast\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            carries two UDP-glucose epimerase genes (\n            <jats:italic>UGE1<\/jats:italic>\n            and\n            <jats:italic>UGE2<\/jats:italic>\n            ).\n            <jats:italic>UGE2<\/jats:italic>\n            maps within a galactose cluster composed of a galactokinase homologue gene and a galactose-1-phosphate uridylyltransferase. This clustered organization of the\n            <jats:italic>GAL<\/jats:italic>\n            genes is similar to that in most of the hemiascomycete yeast genomes and in\n            <jats:italic>Schizosaccharomyces pombe<\/jats:italic>\n            but is otherwise not generally conserved in the fungal kingdom.\n            <jats:italic>UGE1<\/jats:italic>\n            has been identified as necessary for galactoxylomannan biosynthesis and virulence. Here, we show that\n            <jats:italic>UGE2<\/jats:italic>\n            is necessary for\n            <jats:italic>C. neoformans<\/jats:italic>\n            cells to utilize galactose as a carbon source at 30\u00b0C but is not required for virulence. In contrast, deletion of\n            <jats:italic>UGE1<\/jats:italic>\n            does not affect cell growth on galactose at this temperature. At 37\u00b0C, a\n            <jats:italic>uge2\u0394<\/jats:italic>\n            mutant grows on galactose in a\n            <jats:italic>UGE1<\/jats:italic>\n            -dependent manner. This compensation by\n            <jats:italic>UGE1<\/jats:italic>\n            of\n            <jats:italic>UGE2<\/jats:italic>\n            mutation for growth on galactose at 37\u00b0C was not associated with upregulation of\n            <jats:italic>UGE1<\/jats:italic>\n            transcription or with an increase of the affinity of the enzyme for UDP-galactose at this temperature. We studied the subcellular localization of the two enzymes. Whereas at 30\u00b0C, Uge1p is at least partially associated with intracellular vesicles and Uge2p is on the plasma membrane, in cells growing on galactose at 37\u00b0C, Uge1p colocalizes with Uge2p to the plasma membrane, suggesting that its activity is regulated through subcellular localization.\n          <\/jats:p>","DOI":"10.1128\/ec.00189-08","type":"journal-article","created":{"date-parts":[[2008,9,27]],"date-time":"2008-09-27T00:45:01Z","timestamp":1222476301000},"page":"2069-2077","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["<i>UGE1<\/i>\n            and\n            <i>UGE2<\/i>\n            Regulate the UDP-Glucose\/UDP-Galactose Equilibrium in\n            <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1128","volume":"7","author":[{"given":"Fre\u0301de\u0301rique","family":"Moyrand","sequence":"first","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS, URA3012, F-75015, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Lafontaine","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire des Levures, F-75015, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 des Aspergillus, F-75015, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Janbon","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS, URA3012, F-75015, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512727200"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.4.655-663.2003"},{"key":"e_1_3_2_5_2","unstructured":"Cryptococcus neoformans. 1998"},{"key":"e_1_3_2_6_2","first-page":"2119","volume":"6","year":"2007","unstructured":"Casadevall, A., and L. 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