{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:16:52Z","timestamp":1790857012477,"version":"4.1.0"},"reference-count":76,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides brasiliensis<\/jats:named-content>\n            is a thermodimorphic human pathogenic fungus that causes paracoccidioidomycosis (PCM), which is the most prevalent systemic mycosis in Latin America. Differentiation from the mycelial to the yeast form (M-to-Y) is an essential step for the establishment of PCM. We evaluated the involvement of mitochondria and intracellular oxidative stress in M-to-Y differentiation. M-to-Y transition was delayed by the inhibition of mitochondrial complexes III and IV or alternative oxidase (AOX) and was blocked by the association of AOX with complex III or IV inhibitors. The expression of\n            <jats:named-content content-type=\"genus-species\">P. brasiliensis<\/jats:named-content>\n            <jats:italic>aox<\/jats:italic>\n            (\n            <jats:italic>Pbaox<\/jats:italic>\n            ) was developmentally regulated through M-to-Y differentiation, wherein the highest levels were achieved in the first 24 h and during the yeast exponential growth phase;\n            <jats:italic>Pbaox<\/jats:italic>\n            was upregulated by oxidative stress.\n            <jats:italic>Pbaox<\/jats:italic>\n            was cloned, and its heterologous expression conferred cyanide-resistant respiration in\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            and reduced oxidative stress in\n            <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n            cells. These results reinforce the role of\n            <jats:italic>Pb<\/jats:italic>\n            AOX in intracellular redox balancing and demonstrate its involvement, as well as that of other components of the mitochondrial respiratory chain complexes, in the early stages of the M-to-Y differentiation of\n            <jats:named-content content-type=\"genus-species\">P. brasiliensis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/ec.00194-10","type":"journal-article","created":{"date-parts":[[2010,12,24]],"date-time":"2010-12-24T04:01:15Z","timestamp":1293163275000},"page":"237-248","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Involvement of an Alternative Oxidase in Oxidative Stress and Mycelium-to-Yeast Differentiation in Paracoccidioides brasiliensis"],"prefix":"10.1128","volume":"10","author":[{"given":"Vicente P.","family":"Martins","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisa M.","family":"Dinamarco","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico M.","family":"Soriani","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria G.","family":"Tudella","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"},{"name":"Laborat\u00f3rio Nacional de Ci\u00eancia e Tecnologia do Bioetanol (CTBE), S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Curti","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica e Qu\u00edmica, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio A.","family":"Uyemura","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycres.2008.07.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00676.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10540-005-2889-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.8.2289-2294.1989"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1500"},{"key":"e_1_3_3_9_2","first-page":"65","article-title":"The respiratory chain and oxidative phosphorylation","volume":"17","author":"Chance B.","year":"1956","unstructured":"ChanceB. WilliamsG. R.. 1956. The respiratory chain and oxidative phosphorylation. Adv. Enzymol. Relat. Subj. Biochem. 17:65\u2013134.","journal-title":"Adv. Enzymol. Relat. Subj. Biochem."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701670509"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2010.07.006"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500625200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-006-0154-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.2.136-169.1997"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693780903055673"},{"key":"e_1_3_3_16_2","first-page":"1091","article-title":"Reactive oxygen species in regulation of fungal development","volume":"72","author":"Gessler N. N.","year":"2007","unstructured":"GesslerN. N. Aver'yanovA. A. BelozerskayaT. A.. 2007. Reactive oxygen species in regulation of fungal development. Biochemistry 72:1091\u20131109.","journal-title":"Biochemistry"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/02656730310001619505"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.1.34-48.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)57021-6"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11554"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/geronj\/11.3.298"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.7124"},{"key":"e_1_3_3_23_2","first-page":"24","article-title":"Aging and oxidative stress","volume":"10","author":"Harman D","year":"1998","unstructured":"HarmanD. 1998. Aging and oxidative stress. J. Int. Fed. Clin. Chem. 10:24\u201327.","journal-title":"J. Int. Fed. Clin. Chem."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(02)00308-0"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3560595"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03734-1"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.05.013"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.968"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00251-6"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00162-6"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/35083594"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.5.657-662.2002"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-008-9191-5"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00716.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.06.009"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.7.4596"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.138.2.647-649.1979"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-004-1016-6"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-008-9163-9"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050346e"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.9.4055-4060.1987"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3001938"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02109999"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1077"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124105"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00120-0"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.12.2115-2128.2005"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00599247"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2007.11.005"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.59.030804.121055"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362178585380481"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.12.2.279-281.1980"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.3.233.241"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.11.002"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-006-9012-7"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)82052-3"},{"key":"e_1_3_3_59_2","unstructured":"SambrookJ.\nFritschE. F.\nManiatisT.. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY."},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219480000501"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.40.3.225.242"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2005000700003"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00340712"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030846"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.10.008"},{"key":"e_1_3_3_66_2","first-page":"383","article-title":"Molecular identification of Paracoccidioides brasiliensis by 5\u2032 nuclease assay. Diagn. Microbiol","volume":"44","author":"Semighini C. P.","year":"2002","unstructured":"SemighiniC. P. de CamargoZ. P. PucciaR. GoldmanM. H. GoldmanG. H.. 2002. Molecular identification of Paracoccidioides brasiliensis by 5\u2032 nuclease assay. Diagn. Microbiol. Infect. Dis. 44:383\u2013386.","journal-title":"Infect. Dis."},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.7.821"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00181-X"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1280"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.01.004"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2005.00050.x"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.01.024"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.09.004"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00194-8"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.035956"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.48.1.703"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(03)00036-9"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00194-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00194-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T15:21:17Z","timestamp":1645629677000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.00194-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":76,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1128\/EC.00194-10"],"URL":"https:\/\/doi.org\/10.1128\/ec.00194-10","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"2010-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-02-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}