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In this work, we survey the version 3.0 draft genome to identify a ferrireductase, FRE1, and two ZIP family proteins, IRT1 and IRT2, as candidate ferrous transporters based on their increased expression in iron-deficient versus iron-replete cells. In a parallel proteomic approach, we identified FEA1 and FEA2 as the major proteins secreted by iron-deficient\n            <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n            . The recovery of FEA1 and FEA2 from the medium of\n            <jats:italic>Chlamydomonas<\/jats:italic>\n            strain CC425 cultures is strictly correlated with iron nutrition status, and the accumulation of the corresponding mRNAs parallels that of the\n            <jats:italic>Chlamydomonas FOX1<\/jats:italic>\n            and\n            <jats:italic>FTR1<\/jats:italic>\n            mRNAs, although the magnitude of regulation is more dramatic for the\n            <jats:italic>FEA<\/jats:italic>\n            genes. Like the\n            <jats:italic>FOX1<\/jats:italic>\n            and\n            <jats:italic>FTR1<\/jats:italic>\n            genes, the\n            <jats:italic>FEA<\/jats:italic>\n            genes do not respond to copper, zinc, or manganese deficiency. The 5\u2032 flanking untranscribed sequences from the\n            <jats:italic>FEA1<\/jats:italic>\n            ,\n            <jats:italic>FTR1<\/jats:italic>\n            , and\n            <jats:italic>FOX1<\/jats:italic>\n            genes confer iron deficiency-dependent expression of\n            <jats:italic>ARS2<\/jats:italic>\n            , suggesting that the iron assimilation pathway is under transcriptional control by iron nutrition. Genetic analysis suggests that the secreted proteins FEA1 and FEA2 facilitate high-affinity iron uptake, perhaps by concentrating iron in the vicinity of the cell. Homologues of\n            <jats:italic>FEA1<\/jats:italic>\n            and\n            <jats:italic>FRE1<\/jats:italic>\n            were identified previously as high-CO\n            <jats:sub>2<\/jats:sub>\n            -responsive genes,\n            <jats:italic>HCR1<\/jats:italic>\n            and\n            <jats:italic>HCR2<\/jats:italic>\n            , in\n            <jats:italic>Chlorococcum littorale<\/jats:italic>\n            , suggesting that components of the iron assimilation pathway are responsive to carbon nutrition. These iron response components are placed in a proposed iron assimilation pathway for\n            <jats:italic>Chlamydomonas<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/ec.00205-07","type":"journal-article","created":{"date-parts":[[2007,7,28]],"date-time":"2007-07-28T01:09:50Z","timestamp":1185584990000},"page":"1841-1852","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":129,"title":["<i>FEA1<\/i>\n            ,\n            <i>FEA2<\/i>\n            , and\n            <i>FRE1<\/i>\n            , Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with\n            <i>FOX1<\/i>\n            and\n            <i>FTR1<\/i>\n            in Iron-Deficient\n            <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1128","volume":"6","author":[{"given":"Michael D.","family":"Allen","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, UCLA, Box 951569, Los Angeles, California 90095-1569"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 A.","family":"del Campo","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, UCLA, Box 951569, Los Angeles, California 90095-1569"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janette","family":"Kropat","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, UCLA, Box 951569, Los Angeles, California 90095-1569"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabeeha S.","family":"Merchant","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, UCLA, Box 951569, Los Angeles, California 90095-1569"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000713200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.088609"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90346-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.401"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3380499"},{"key":"e_1_3_2_7_2","first-page":"163","volume":"193","year":"1994","unstructured":"Benderliev, K. 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