{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:20:27Z","timestamp":1778862027144,"version":"3.51.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Blastomyces dermatitidis<\/jats:named-content>is a dimorphic fungal pathogen that primarily causes blastomycosis in the midwestern and northern United States and Canada. While the genes controlling sexual development have been known for a long time, the genes controlling sexual reproduction of<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>(teleomorph,<jats:named-content content-type=\"genus-species\">Ajellomyces dermatitidis<\/jats:named-content>) are unknown. We identified the mating-type (<jats:italic>MAT<\/jats:italic>) locus in the<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>genome by comparative genomic approaches. The<jats:named-content content-type=\"genus-species\">B. dermatitidis MAT<\/jats:named-content>locus resembles those of other dimorphic fungi, containing either an alpha-box (<jats:italic>MAT1-1<\/jats:italic>) or an HMG domain (<jats:italic>MAT1-2<\/jats:italic>) gene linked to the<jats:italic>APN2<\/jats:italic>,<jats:italic>SLA2<\/jats:italic>, and<jats:italic>COX13<\/jats:italic>genes. However, in some strains of<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>, the<jats:italic>MAT<\/jats:italic>locus harbors transposable elements (TEs) that make it unusually large compared to the<jats:italic>MAT<\/jats:italic>locus of other dimorphic fungi. Based on the<jats:italic>MAT<\/jats:italic>locus sequences of<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>, we designed specific primers for PCR determination of the mating type. Two<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>isolates of opposite mating types were cocultured on mating medium. Immature sexual structures were observed starting at 3 weeks of coculture, with coiled-hyphae-containing cleistothecia developing over the next 3 to 6 weeks. Genetic recombination was detected in potential progeny by mating-type determination, PCR-restriction fragment length polymorphism (PCR-RFLP), and random amplification of polymorphic DNA (RAPD) analyses, suggesting that a meiotic sexual cycle might have been completed. The F1 progeny were sexually fertile when tested with strains of the opposite mating type. Our studies provide a model for the evolution of the<jats:italic>MAT<\/jats:italic>locus in the dimorphic and closely related fungi and open the door to classic genetic analysis and studies on the possible roles of mating and mating type in infection and virulence.<\/jats:p>","DOI":"10.1128\/ec.00249-12","type":"journal-article","created":{"date-parts":[[2012,11,11]],"date-time":"2012-11-11T01:35:03Z","timestamp":1352597703000},"page":"109-117","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Identification of the Mating-Type (<i>MAT<\/i>) Locus That Controls Sexual Reproduction of Blastomyces dermatitidis"],"prefix":"10.1128","volume":"12","author":[{"given":"Wenjun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas D.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Medicine, and Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Walton","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Floyd","family":"Averette","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharadha","family":"Sakthikumar","sequence":"additional","affiliation":[{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina A.","family":"Cuomo","sequence":"additional","affiliation":[{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce S.","family":"Klein","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Medicine, and Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Heitman","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.04.002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408418209104489"},{"key":"e_1_3_2_4_2","first-page":"29","article-title":"Epidemiologic aspects of blastomycosis, the enigmatic systemic mycosis","volume":"1","author":"Klein BS","year":"1986","unstructured":"KleinBS VergerontJM DavisJP. 1986. 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