{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:35:08Z","timestamp":1784763308987,"version":"3.55.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Msi1-like (MSIL) proteins contain WD40 motifs and have a pleiotropic cellular function as negative regulators of the Ras\/cyclic AMP (cAMP) pathway and components of chromatin assembly factor 1 (CAF-1), yet they have not been studied in fungal pathogens. Here we identified and characterized an MSIL protein, Msl1, in\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            , which causes life-threatening meningoencephalitis in humans. Notably, Msl1 plays pleiotropic roles in\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            in both cAMP-dependent and -independent manners largely independent of Ras. Msl1 negatively controls antioxidant melanin production and sexual differentiation, and this was repressed by the inhibition of the cAMP-signaling pathway. In contrast, Msl1 controls thermotolerance, diverse stress responses, and antifungal drug resistance in a Ras\/cAMP-independent manner. Cac2, which is the second CAF-1 component, appears to play both redundant and distinct functions compared to the functions of Msl1. Msl1 is required for the full virulence of\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            . Transcriptome analysis identified a group of Msl1-regulated genes, which include stress-related genes such as\n            <jats:italic>HSP12<\/jats:italic>\n            and\n            <jats:italic>HSP78<\/jats:italic>\n            . In conclusion, this study demonstrates pleiotropic roles of Msl1 in the human fungal pathogen\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            , providing insight into a potential novel antifungal therapeutic target.\n          <\/jats:p>","DOI":"10.1128\/ec.00261-12","type":"journal-article","created":{"date-parts":[[2012,10,6]],"date-time":"2012-10-06T06:08:45Z","timestamp":1349503725000},"page":"1482-1495","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Pleiotropic Roles of the Msi1-Like Protein Msl1 in Cryptococcus neoformans"],"prefix":"10.1128","volume":"11","author":[{"given":"Dong-Hoon","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinae","family":"Maeng","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K.","family":"Strain","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Floyd","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Heitman","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Sun","family":"Bahn","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01852.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.23.3206"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1998.1079"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.1.75-84.2002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.09.047"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1476-1491.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-11-0987"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1578"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00103-X"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-8-2607"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202755200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.9.3179-3191.2001"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.3.358"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/151.2.485"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112000"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.04.004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.12.1971-1981.2005"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.1.14-26.2004"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.8.2924-2935.2006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.708"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.5.1784-1794.2001"},{"key":"e_1_3_3_23_2","doi-asserted-by":"crossref","unstructured":"JullienPE. 2008. 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