{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:19:16Z","timestamp":1791105556067,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Dictyostelium discoideum<\/jats:named-content>\n            amoebae have been used extensively to study the structure and dynamics of the endocytic pathway. Here, we show that while the general structure of the endocytic pathway is maintained in starved cells, its dynamics rapidly slow down. In addition, analysis of\n            <jats:italic>apm3<\/jats:italic>\n            and\n            <jats:italic>lvsB<\/jats:italic>\n            mutants reveals that the functional organization of the endocytic pathway is profoundly modified upon starvation. Indeed, in these mutant cells, some of the defects observed in rich medium persist in starved cells, notably an abnormally slow transfer of endocytosed material between endocytic compartments. Other parameters, such as endocytosis of the fluid phase or the rate of fusion of postlysosomes to the cell surface, vary dramatically upon starvation. Studying the endocytic pathway in starved cells can provide a different perspective, allowing the primary (invariant) defects resulting from specific mutations to be distinguished from their secondary (conditional) consequences.\n          <\/jats:p>","DOI":"10.1128\/ec.00285-09","type":"journal-article","created":{"date-parts":[[2010,1,23]],"date-time":"2010-01-23T02:07:24Z","timestamp":1264212444000},"page":"387-392","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Effect of Starvation on the Endocytic Pathway in Dictyostelium Cells"],"prefix":"10.1128","volume":"9","author":[{"given":"Ewan W.","family":"Smith","sequence":"first","affiliation":[{"name":"Dept. for Cell Physiology and Metabolism, Centre M\u00e9dical Universitaire, Geneva Faculty of Medicine, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanessa C.","family":"Lima","sequence":"additional","affiliation":[{"name":"Dept. for Cell Physiology and Metabolism, Centre M\u00e9dical Universitaire, Geneva Faculty of Medicine, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve J.","family":"Charette","sequence":"additional","affiliation":[{"name":"Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec (H\u00f4pital Laval), 2725 Chemin Sainte-Foy, Quebec City, Quebec, Canada G1V 4G5; and"},{"name":"D\u00e9partement de Biochimie et Microbiologie, Facult\u00e9 des Sciences et G\u00e9nie, Universit\u00e9 Laval, Quebec City, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Cosson","sequence":"additional","affiliation":[{"name":"Dept. for Cell Physiology and Metabolism, Centre M\u00e9dical Universitaire, Geneva Faculty of Medicine, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510000279"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42288-5"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.009"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.009001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00706.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00478.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-07-0619"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.4.737"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006725200"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00545-X"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69026-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00299.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-04-0206"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-09-0454"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e3282f2bcce"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00567.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2003.40101.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01524"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03190"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-08-0392"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1998.3971"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00215"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10254"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00285-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00285-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:50:29Z","timestamp":1645627829000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.00285-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1128\/EC.00285-09"],"URL":"https:\/\/doi.org\/10.1128\/ec.00285-09","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3]]},"assertion":[{"value":"2009-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}