{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T06:23:30Z","timestamp":1782627810943,"version":"3.54.5"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Small-subunit (SSU) rRNA-based methods have been commonly used in the differentiation of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species or genotypes. In order to develop a new tool for confirming the genotypes of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species, parts of the 90-kDa heat shock protein (Hsp90) genes of seven\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species and genotypes known to infect humans (\n            <jats:italic>C. hominis<\/jats:italic>\n            ,\n            <jats:italic>C. parvum<\/jats:italic>\n            ,\n            <jats:italic>C. meleagridis<\/jats:italic>\n            ,\n            <jats:italic>C. canis<\/jats:italic>\n            ,\n            <jats:italic>C. muris<\/jats:italic>\n            ,\n            <jats:italic>C. suis<\/jats:italic>\n            , and the cervine genotype), together with one from cattle (\n            <jats:italic>C. andersoni<\/jats:italic>\n            ), were sequenced and analyzed. With the exception of\n            <jats:italic>C. felis<\/jats:italic>\n            from cats and\n            <jats:italic>C. baileyi<\/jats:italic>\n            from birds, the Hsp90 genes of all tested\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species were amplified. Phylogenetic analysis of the\n            <jats:italic>hsp90<\/jats:italic>\n            sequences from all these species is congruent with previous studies in which the SSU rRNA, 70-kDa heat shock protein, oocyst wall protein, and actin genes were analyzed and showed that gastric and intestinal parasites segregate into two distinct clades. In this study, the secondary products of\n            <jats:italic>hsp90<\/jats:italic>\n            produced after PCR-restriction fragment length digestion with StyI and HphI or with BbsI showed that parasites within the intestinal or gastric clade could be differentiated from each other. These data confirm the utility of the Hsp90 gene as a sensitive, specific, and robust molecular tool for differentiating species and\/or genotypes of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            in clinical specimens.\n          <\/jats:p>","DOI":"10.1128\/ec.00294-08","type":"journal-article","created":{"date-parts":[[2009,1,24]],"date-time":"2009-01-24T02:49:55Z","timestamp":1232765395000},"page":"478-482","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["90-Kilodalton Heat Shock Protein, Hsp90, as a Target for Genotyping\n            <i>Cryptosporidium<\/i>\n            spp. Known To Infect Humans"],"prefix":"10.1128","volume":"8","author":[{"given":"Yaoyu","family":"Feng","sequence":"first","affiliation":[{"name":"School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Dearen","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaliano","family":"Cama","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094786"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6748-6752.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000077714"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1017-1023.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.13429"},{"key":"e_1_3_2_7_2","first-page":"1","year":"2008","unstructured":"Fayer, R. 2008. Introduction, p. 1-42. In R. Fayer and L. Xiao (ed.), Cryptosporidium and cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.","journal-title":"Cryptosporidium and cryptosporidiosis"},{"key":"e_1_3_2_8_2","first-page":"1","year":"1997","unstructured":"Fayer, R., C. A. Speer, and J. P. Dubey. 1997. The general biology of Cryptosporidium, p. 1-41. In R. Fayer (ed.), Cryptosporidium and cryptosporidiosis. 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A., and R. L. Guerrant. 2008. Clinical disease and pathology, p. 235-253. In R. Fayer and L. Xiao (ed.), Cryptosporidium and cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.","journal-title":"Cryptosporidium and cryptosporidiosis"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.4.1578-1583.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.72-97.2004"},{"key":"e_1_3_2_26_2","first-page":"44S","volume":"46","year":"1999","unstructured":"Xiao, L., J. R. Limor, L. Li, U. Morgan, R. C. Thompson, and A. A. Lal. 1999. Presence of heterogeneous copies of the small subunit rRNA gene in Cryptosporidium parvum human and marsupial genotypes and Cryptosporidium felis. J. Eukaryot Microbiol.46:44S-45S.","journal-title":"J. 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