{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:08Z","timestamp":1791196748270,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The apicomplexan parasite\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            is a significant cause of diarrheal disease worldwide. Previously, we reported that a\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            subtilisin-like serine protease activity with furin-type specificity cleaves gp40\/15, a glycoprotein that is proteolytically processed into gp40 and gp15, which are implicated in mediating infection of host cells. Neither the enzyme(s) responsible for the protease activity in\n            <jats:italic>C. parvum<\/jats:italic>\n            lysates nor those that process gp40\/15 are known. There are no furin or other proprotein convertase genes in the\n            <jats:italic>C. parvum<\/jats:italic>\n            genome. However, a gene encoding CpSUB1, a subtilisin-like serine protease, is present. In this study, we cloned the CpSUB1 genomic sequence and expressed and purified the recombinant prodomain. Reverse transcriptase PCR analysis of RNA from\n            <jats:italic>C. parvum<\/jats:italic>\n            -infected HCT-8 cells revealed that CpSUB1 is expressed throughout infection in vitro. In immunoblots, antiserum to the recombinant CpSUB1 prodomain revealed two major bands, of \u223c64 kDa and \u223c48 kDa, for\n            <jats:italic>C. parvum<\/jats:italic>\n            lysates and proteins \u201cshed\u201d during excystation. In immunofluorescence assays, the antiserum reacted with the apical region of sporozoites and merozoites. The recombinant prodomain inhibited protease activity and processing of recombinant gp40\/15 by\n            <jats:italic>C. parvum<\/jats:italic>\n            lysates but not by furin. Since prodomains are often selective inhibitors of their cognate enzymes, these results suggest that CpSUB1 may be a likely candidate for the protease activity in\n            <jats:italic>C. parvum<\/jats:italic>\n            and for processing of gp40\/15. Importantly, the recombinant prodomain inhibited\n            <jats:italic>C. parvum<\/jats:italic>\n            infection of HCT-8 cells. These studies indicate that CpSUB1 plays a significant role in infection of host cells by the parasite and suggest that this enzyme may serve as a target for intervention.\n          <\/jats:p>","DOI":"10.1128\/ec.00306-08","type":"journal-article","created":{"date-parts":[[2009,1,24]],"date-time":"2009-01-24T02:49:55Z","timestamp":1232765395000},"page":"470-477","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Role of CpSUB1, a Subtilisin-Like Protease, in\n            <i>Cryptosporidium parvum<\/i>\n            Infection In Vitro"],"prefix":"10.1128","volume":"8","author":[{"given":"Jane W.","family":"Wanyiri","sequence":"first","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center, 800 Washington St., Boston, MA 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patsharaporn","family":"Techasintana","sequence":"additional","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center, 800 Washington St., Boston, MA 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta M.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center, 800 Washington St., Boston, MA 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Blackman","sequence":"additional","affiliation":[{"name":"Division of Parasitology, The National Institute for Medical Research, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kami","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Immunology and Microbiology, Albert Einstein College of Medicine, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honorine D.","family":"Ward","sequence":"additional","affiliation":[{"name":"Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center, 800 Washington St., Boston, MA 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094786"},{"key":"e_1_3_2_3_2","first-page":"CD004932","volume":"2007","year":"2007","unstructured":"Abubakar, I., S. 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