{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:46:13Z","timestamp":1790063173901,"version":"4.0.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The mitochondrial DNA of trypanosomes contains two types of circular DNAs, minicircles and maxicircles. Both minicircles and maxicircles replicate from specific replication origins by unidirectional theta-type intermediates. Initiation of the minicircle leading strand and also that of at least the first Okazaki fragment involve RNA priming. The\n            <jats:named-content content-type=\"genus-species\">Trypanosoma brucei<\/jats:named-content>\n            genome encodes two mitochondrial DNA primases, PRI1 and PRI2, related to the primases of eukaryotic nucleocytoplasmic large DNA viruses. These primases are members of the archeoeukaryotic primase superfamily, and each of them contain an RNA recognition motif and a PriCT-2 motif. In\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            species, PRI2 proteins are approximately 61 to 66 kDa in size, whereas in\n            <jats:named-content content-type=\"genus-species\">Trypanosoma<\/jats:named-content>\n            species, PRI2 proteins have additional long amino-terminal extensions. RNA interference (RNAi) of\n            <jats:italic>T. brucei PRI2<\/jats:italic>\n            resulted in the loss of kinetoplast DNA and accumulation of covalently closed free minicircles. Recombinant PRI2 lacking this extension (PRI2\u0394NT) primes poly(dA) synthesis on a poly(dT) template in an ATP-dependent manner. Mutation of two conserved aspartate residues (PRI2\u0394NTCS) resulted in loss of enzymatic activity but not loss of DNA binding. We propose that PRI2 is directly involved in initiating kinetoplast minicircle replication.\n          <\/jats:p>","DOI":"10.1128\/ec.00308-10","type":"journal-article","created":{"date-parts":[[2011,1,22]],"date-time":"2011-01-22T05:07:11Z","timestamp":1295672831000},"page":"445-454","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["A Second Mitochondrial DNA Primase Is Essential for Cell Growth and Kinetoplast Minicircle DNA Replication in Trypanosoma brucei"],"prefix":"10.1128","volume":"10","author":[{"given":"Jane C.","family":"Hines","sequence":"first","affiliation":[{"name":"Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California 90095-1570"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan S.","family":"Ray","sequence":"additional","affiliation":[{"name":"Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California 90095-1570"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.13.12.7766-7773.1993"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.4.725"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35945-8"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.12.6794"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1977.tb00987.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.4.765-774.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.4.735"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.20.4733"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.3.725"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.15.8455"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)30096-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90431-B"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.12.021"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200662200"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00272-9"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01231-09"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki702"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.4.911"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161455"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83701-8"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42581-6"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1078\/1434-4610-00066"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00571-3"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00284-07"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2005.06.008"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00369-06"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.07.004"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000589"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.084038"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.4.495-502.2002"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90252-8"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-08-0525"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606374200"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.2.277-287.2004"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.5.1069"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85016-0"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.9.8.3212-3217.1989"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308565200"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06087.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7809"},{"key":"e_1_3_3_42_2","first-page":"169","article-title":"Early intermediates in the replication of DNA minicircles in isolated kinetoplasts from Crithidia fasciculata","volume":"9","author":"Sheline C.","year":"1988","unstructured":"ShelineC. 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