{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:33:09Z","timestamp":1784590389307,"version":"3.55.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic>capsular polysaccharide is composed of at least two components, glucuronoxylomannan (GXM) and galactoxylomannans (GalXM). Although GXM has been extensively studied, little is known about the location of GalXM in the<jats:italic>C. neoformans<\/jats:italic>capsule, in part because there are no serological reagents specific to this antigen. To circumvent the poor immunogenicity of GalXM, this antigen was conjugated to protective antigen from<jats:italic>Bacillus anthracis<\/jats:italic>as a protein carrier. The resulting conjugate elicited antibodies that reacted with GalXM in mice and yielded an immune serum that proved useful for studying GalXM in the polysaccharide capsule. In acapsular cells, immune serum localized GalXM to the cell wall. In capsulated cells, immune serum localized GalXM to discrete pockets near the capsule edge. GalXM was abundant on the nascent capsules of budding daughter cells. The constituent sugars of GalXM were found in vesicle fractions consistent with vesicular transport for this polysaccharide. In addition, we generated a single-chain fraction variable fragment antibody with specificity to oxidized carbohydrates that also produced punctate immunofluorescence on encapsulated cells that partially colocalized with GalXM. The results are interpreted to mean that GalXM is a transient component of the polysaccharide capsule of mature cells during the process of secretion. Hence, the function of GalXM appears to be more consistent with that of an exopolysaccharide than a structural component of the cryptococcal capsule.<\/jats:p>","DOI":"10.1128\/ec.00331-08","type":"journal-article","created":{"date-parts":[[2008,10,25]],"date-time":"2008-10-25T01:36:26Z","timestamp":1224898586000},"page":"96-103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Capsular Localization of the<i>Cryptococcus neoformans<\/i>Polysaccharide Component Galactoxylomannan"],"prefix":"10.1128","volume":"8","author":[{"given":"Magdia","family":"De Jesus","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301 Moraes","family":"Nicola","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio L.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Estudos Integrados em Bioqui\u0301mica Microbiana, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941590, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Janbon","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS, URA3012 F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00221-0"},{"key":"e_1_3_2_3_2","unstructured":"Phage display: a laboratory manual. 2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.4.655-663.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.2.465-475.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90209-C"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.59-64.1991"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)80686-2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.5.1507-1512.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00368-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701747182"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.10.3700-3707.1991"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.8.1895-1899.1987"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"914","DOI":"10.4049\/jimmunol.98.5.914","volume":"98","year":"1967","unstructured":"Goren, M. 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