{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:03:08Z","timestamp":1770476588947,"version":"3.49.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Trypanosomatid parasites share a gene expression mode which differs greatly from that of their human and insect hosts. In these unicellular eukaryotes, protein-coding genes are transcribed polycistronically and individual mRNAs are processed from precursors by spliced leader (SL)\n            <jats:italic>trans<\/jats:italic>\n            splicing and polyadenylation. In\n            <jats:italic>trans<\/jats:italic>\n            splicing, the SL RNA is consumed through a transfer of its 5\u2032-terminal part to the 5\u2032 end of mRNAs. Since all mRNAs are\n            <jats:italic>trans<\/jats:italic>\n            spliced, the parasites depend on strong and continuous SL RNA synthesis mediated by RNA polymerase II. As essential factors for SL RNA gene transcription in\n            <jats:italic>Trypanosoma brucei<\/jats:italic>\n            , the general transcription factor (GTF) IIB and a complex, consisting of the TATA-binding protein-related protein 4, the small nuclear RNA-activating protein complex, and TFIIA, were recently identified. Although\n            <jats:italic>T. brucei<\/jats:italic>\n            TFIIA and TFIIB are extremely divergent to their counterparts in other eukaryotes, their characterization suggested that trypanosomatids do form a class II transcription preinitiation complex at the SL RNA gene promoter and harbor orthologues of other known GTFs. TFIIH is a GTF which functions in transcription initiation, DNA repair, and cell cycle control. Here, we investigated whether a\n            <jats:italic>T. brucei<\/jats:italic>\n            TFIIH is important for SL RNA gene transcription and found that silencing the expression of the highly conserved TFIIH subunit XPD in\n            <jats:italic>T. brucei<\/jats:italic>\n            affected SL RNA gene synthesis in vivo, and depletion of this protein from extract abolished SL RNA gene transcription in vitro. Since we also identified orthologues of the TFIIH subunits XPB, p52\/TFB2, and p44\/SSL1 copurifying with TbXPD, we concluded that the parasite harbors a TFIIH which is indispensable for SL RNA gene transcription.\n          <\/jats:p>","DOI":"10.1128\/ec.00411-06","type":"journal-article","created":{"date-parts":[[2007,1,27]],"date-time":"2007-01-27T01:43:40Z","timestamp":1169862220000},"page":"641-649","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Spliced Leader RNA Gene Transcription in\n            <i>Trypanosoma brucei<\/i>\n            Requires Transcription Factor TFIIH"],"prefix":"10.1128","volume":"6","author":[{"given":"Ju Huck","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Genetics and Developmental Biology and Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-3301"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu N.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Genetics and Developmental Biology and Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-3301"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Schimanski","sequence":"additional","affiliation":[{"name":"Department of Genetics and Developmental Biology and Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-3301"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Gu\u0308nzl","sequence":"additional","affiliation":[{"name":"Department of Genetics and Developmental Biology and Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 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J., and A. Tait. 1996. Trypanosomatid genetics, p. 6-34. In D. F. Smith and M. Parsons (ed.), Molecular biology of parasitic protozoa. Oxford University Press, Oxford, United Kingdom.","journal-title":"Molecular biology of parasitic protozoa"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.11.3319"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00002-X"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47222-5"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(98)00083-8"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2003.08.005"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00411-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.00411-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T14:15:43Z","timestamp":1645625743000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.00411-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/EC.00411-06"],"URL":"https:\/\/doi.org\/10.1128\/ec.00411-06","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-01-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}