{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:20:00Z","timestamp":1791181200985,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A gene predicted to encode\n                    <jats:named-content content-type=\"genus-species\">Trypanosoma brucei<\/jats:named-content>\n                    glucosamine 6-phosphate\n                    <jats:italic>N<\/jats:italic>\n                    -acetyltransferase (\n                    <jats:italic>TbGNA1<\/jats:italic>\n                    ; EC 2.3.1.4) was cloned and expressed in\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    . The recombinant protein was enzymatically active, and its high-resolution crystal structure was obtained at 1.86 \u00c5. Endogenous TbGNA1 protein was localized to the peroxisome-like microbody, the glycosome. A bloodstream-form\n                    <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n                    <jats:italic>GNA1<\/jats:italic>\n                    conditional null mutant was constructed and shown to be unable to sustain growth\n                    <jats:italic>in vitro<\/jats:italic>\n                    under nonpermissive conditions, demonstrating that there are no metabolic or nutritional routes to UDP-GlcNAc other than via GlcNAc-6-phosphate. Analysis of the protein glycosylation phenotype of the\n                    <jats:italic>TbGNA1<\/jats:italic>\n                    mutant under nonpermissive conditions revealed that poly-\n                    <jats:italic>N<\/jats:italic>\n                    -acetyllactosamine structures were greatly reduced in the parasite and that the glycosylation profile of the principal parasite surface coat component, the variant surface glycoprotein (VSG), was modified. The significance of results and the potential of TbGNA1 as a novel drug target for African sleeping sickness are discussed.\n                  <\/jats:p>","DOI":"10.1128\/ec.05025-11","type":"journal-article","created":{"date-parts":[[2011,4,29]],"date-time":"2011-04-29T23:10:45Z","timestamp":1304118645000},"page":"985-997","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Characterization, Localization, Essentiality, and High-Resolution Crystal Structure of Glucosamine 6-Phosphate\n                    <i>N<\/i>\n                    -Acetyltransferase from Trypanosoma brucei"],"prefix":"10.1128","volume":"10","author":[{"given":"Karina","family":"Mari\u00f1o","sequence":"first","affiliation":[{"name":"Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Lucia Sampaio","family":"G\u00fcther","sequence":"additional","affiliation":[{"name":"Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy K.","family":"Wernimont","sequence":"additional","affiliation":[{"name":"Structural Genomics Consortium, University of Toronto, MaRS South Tower, 7th Floor, 101 College St., Toronto, Ontario, Canada M5G 1L7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Qiu","sequence":"additional","affiliation":[{"name":"Structural Genomics Consortium, University of Toronto, MaRS South Tower, 7th Floor, 101 College St., Toronto, Ontario, Canada M5G 1L7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Hui","sequence":"additional","affiliation":[{"name":"Structural Genomics Consortium, University of Toronto, MaRS South Tower, 7th Floor, 101 College St., Toronto, Ontario, Canada M5G 1L7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A. J.","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.16.3253"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411061200"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2003.09.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.17.12821"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500668"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950180406"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.067223"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312365200"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090896"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)60444-2"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02327"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42479-3"},{"key":"e_1_3_3_16_2","first-page":"47","volume-title":"Essentials of glycobiology","author":"Freeze H. H.","year":"2008","unstructured":"FreezeH. H. ElbeinA. B.. 2008. Glycosylation precursors, p. 47\u201361. In VarkiA. CummingsR. D. EskoJ. D. FreezeH. H. StanleyP. BertozziC. R. HartG. W. EtzlerM. E. (ed.). Essentials of glycobiology, 2nd ed. Cold Spring Harbor Laboratory Press, New York, NY.","edition":"2"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2010.01.002"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm060732n"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806664105"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2520133.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081000"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.10.065"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889893005588"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2005.02.005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444907050172"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.02.011"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn014"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwq115"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00187-4"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.08.019"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1337"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-7-1753"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.1.424"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001245"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030635"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80018-4"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.02.010"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06195.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009988200"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp121"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.222373899"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502370200"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092669999"},{"key":"e_1_3_3_45_2","first-page":"187","article-title":"A model for sedimentation in inhomogeneous media","volume":"108","author":"Schuck P","year":"2004","unstructured":"SchuckP. 2004. A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutes. Biophys. Chem. 108:187\u2013200.","journal-title":"I. Dynamic density gradients from sedimenting co-solutes. Biophys. Chem."},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050442"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709581200"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200111075"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1066"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00175-07"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704742200"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.10.011"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2004.09.003"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.07.040"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00002-X"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5371"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100238"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.05025-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.05025-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T10:23:28Z","timestamp":1645611808000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.05025-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":57,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1128\/EC.05025-11"],"URL":"https:\/\/doi.org\/10.1128\/ec.05025-11","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2011-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}