{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:52:36Z","timestamp":1776732756589,"version":"3.51.2"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We recently demonstrated that\n            <jats:italic>CDR1<\/jats:italic>\n            overexpression in azole-resistant isolates of\n            <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n            is due to its enhanced transcriptional activation and increased mRNA stability. In this study, we provide the first evidence of transcriptional regulation of\n            <jats:italic>CDR1<\/jats:italic>\n            by Ncb2, the \u03b2 subunit of NC2, a heterodimeric regulator of transcription. Conditional\n            <jats:italic>NCB2<\/jats:italic>\n            null mutants displayed decreased susceptibility toward azole and an enhanced transcription of\n            <jats:italic>CDR1<\/jats:italic>\n            . Interestingly, Ncb2 associated with the\n            <jats:italic>CDR1<\/jats:italic>\n            promoter under both repression and activation; however, an increase in recruitment was observed under both transient and constitutive activation states. By chromatin immunoprecipitation (ChIP) assay, we showed the preferential recruitment of Ncb2 to the core TATA region under activation (azole-resistant isolate), while under repression (azole-susceptible isolate) it was present at the TATA upstream region. Further, ChIP analysis revealed that Ncb2 binding was not restricted to the\n            <jats:italic>CDR1<\/jats:italic>\n            gene; instead, it was observed on the promoters of genes coregulated with\n            <jats:italic>CDR1<\/jats:italic>\n            by the transcription activator Tac1. The\n            <jats:italic>tac1<\/jats:italic>\n            \u0394 null mutants, which fail to show the drug-induced transient activation of\n            <jats:italic>CDR1<\/jats:italic>\n            , also showed no increase in Ncb2 recruitment at the promoter. Taken together, our results show that Ncb2, in conjunction with Tac1, is involved in the transcriptional activation of\n            <jats:italic>CDR1<\/jats:italic>\n            , opening up new therapeutic possibilities to combat multidrug resistance (MDR) in\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/ec.05041-11","type":"journal-article","created":{"date-parts":[[2011,8,20]],"date-time":"2011-08-20T02:43:19Z","timestamp":1313808199000},"page":"1357-1366","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Ncb2 Is Involved in Activated Transcription of\n            <i>CDR1<\/i>\n            in Azole-Resistant Clinical Isolates of Candida albicans"],"prefix":"10.1128","volume":"10","author":[{"given":"Shipra","family":"Shukla","sequence":"first","affiliation":[{"name":"Special Centre for Molecular Medicine, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vipin","family":"Yadav","sequence":"additional","affiliation":[{"name":"Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gauranga","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendra","family":"Prasad","sequence":"additional","affiliation":[{"name":"Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703490104"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202236699"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01641.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4505-4512.2004"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.054767"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1639-1652.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.19.6735-6749.2002"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02814.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3145"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.113"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1356(03)00204-6"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.8.2736-2742.2001"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406343200"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.22.4450"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00673.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90172-9"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/npre.2010.4139.1"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1067"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.31.18405"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.3.820"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12910.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01839.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01106-07"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.035154-0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-009-0021-y"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm1078"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90530-C"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.8.1033"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.08.002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02713.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.2.298-309.2005"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152606784112164"},{"key":"e_1_3_3_34_2","first-page":"601","volume-title":"Fungal pathogenesis","author":"Prasad R.","year":"2000","unstructured":"PrasadR. 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