{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T02:53:15Z","timestamp":1777344795773,"version":"3.51.4"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genus\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides<\/jats:named-content>\n            includes the thermodimorphic species\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides brasiliensis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">P. lutzii<\/jats:named-content>\n            , both of which are etiologic agents of paracoccidioidomycosis, a systemic mycosis that affects humans in Latin America. Despite the common occurrence of a sexual stage among closely related fungi, this has not been observed with\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides<\/jats:named-content>\n            species, which have thus been considered asexual. Molecular evolutionary studies revealed recombination events within isolated populations of the genus\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides<\/jats:named-content>\n            , suggesting the possible existence of a sexual cycle. Comparative genomic analysis of all dimorphic fungi and\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            demonstrated the presence of conserved genes involved in sexual reproduction, including those encoding mating regulators such as MAT, pheromone receptors, pheromone-processing enzymes, and mating signaling regulators. The expression of sex-related genes in the yeast and mycelial phases of both\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides<\/jats:named-content>\n            species was also detected by real-time PCR, with nearly all of these genes being expressed preferentially in the filamentous form of the pathogens. In addition, the expression of sex-related genes was responsive to the putative presence of pheromone in the supernatants obtained from previous cocultures of strains of two different mating types.\n            <jats:italic>In vitro<\/jats:italic>\n            crossing of isolates of different mating types, discriminated by phylogenetic analysis of the \u03b1-box (\n            <jats:italic>MAT1-1<\/jats:italic>\n            ) and the high-mobility-group (HMG) domain (\n            <jats:italic>MAT1-2<\/jats:italic>\n            ), led to the identification of the formation of young ascocarps with constricted coiled hyphae related to the initial stage of mating. These genomic and morphological analyses strongly support the existence of a sexual cycle in species of the genus\n            <jats:named-content content-type=\"genus-species\">Paracoccidioides<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/ec.05052-11","type":"journal-article","created":{"date-parts":[[2012,11,3]],"date-time":"2012-11-03T04:27:44Z","timestamp":1351916864000},"page":"380-389","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Molecular and Morphological Data Support the Existence of a Sexual Cycle in Species of the Genus Paracoccidioides"],"prefix":"10.1128","volume":"12","author":[{"given":"Marcus de Melo","family":"Teixeira","sequence":"first","affiliation":[{"name":"Universidade de Bras\u00edlia, Instituto de Ci\u00eancias Biol\u00f3gicas, UNB, Distrito Federal, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel Cordeiro","family":"Theodoro","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Campus de Botucatu, UNESP, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena da Silveira","family":"Derengowski","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia, Instituto de Ci\u00eancias Biol\u00f3gicas, UNB, Distrito Federal, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Moraes","family":"Nicola","sequence":"additional","affiliation":[{"name":"Universidade Cat\u00f3lica de Bras\u00edlia, UCB, Distrito Federal, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Bagagli","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista, Campus de Botucatu, UNESP, S\u00e3o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Sueli","family":"Felipe","sequence":"additional","affiliation":[{"name":"Universidade de Bras\u00edlia, Instituto de Ci\u00eancias Biol\u00f3gicas, UNB, Distrito Federal, Brazil"},{"name":"Universidade Cat\u00f3lica de Bras\u00edlia, UCB, Distrito Federal, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Pleomorphic fungi: the diversity and its taxonomic implications","author":"Sugiyama J","year":"1987","unstructured":"SugiyamaJ . 1987. 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