{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:16:17Z","timestamp":1790144177040,"version":"4.0.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Trypanosoma brucei rhodesiense<\/jats:named-content>\n            is the causative agent of human African sleeping sickness. While the closely related subspecies\n            <jats:named-content content-type=\"genus-species\">T. brucei brucei<\/jats:named-content>\n            is highly susceptible to lysis by a subclass of human high-density lipoproteins (HDL) called trypanosome lytic factor (TLF),\n            <jats:named-content content-type=\"genus-species\">T. brucei rhodesiense<\/jats:named-content>\n            is resistant and therefore able to establish acute and fatal infections in humans. This resistance is due to expression of the serum resistance-associated (SRA) gene, a member of the variant surface glycoprotein (VSG) gene family. Although much has been done to establish the role of SRA in human serum resistance, the specific molecular mechanism of SRA-mediated resistance remains a mystery. Thus, we report the trafficking and steady-state localization of SRA in order to provide more insight into the mechanism of SRA-mediated resistance. We show that SRA traffics to the flagellar pocket of bloodstream-form\n            <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n            organisms, where it localizes transiently before being endocytosed to its steady-state localization in endosomes, and we demonstrate that the critical point of colocalization between SRA and TLF occurs intracellularly.\n          <\/jats:p>","DOI":"10.1128\/ec.05112-11","type":"journal-article","created":{"date-parts":[[2011,6,25]],"date-time":"2011-06-25T05:31:55Z","timestamp":1308979915000},"page":"1023-1033","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Endosomal Localization of the Serum Resistance-Associated Protein in African Trypanosomes Confers Human Infectivity"],"prefix":"10.1128","volume":"10","author":[{"given":"Natalie A.","family":"Stephens","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Hajduk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.14.4285"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2003.07.005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.4.805"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00368-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.04.009"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00306-7"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/302349a0"},{"key":"e_1_3_3_9_2","first-page":"13","article-title":"Only the serum-resistant bloodstream forms of Trypanosoma brucei rhodesiense express the serum resistance associated (SRA) protein","volume":"72","author":"De Greef C.","year":"1992","unstructured":"De GreefC. ChimfwembeE. Kihang'a WabachaJ. Bajyana SongaE. HamersR. 1992. Only the serum-resistant bloodstream forms of Trypanosoma brucei rhodesiense express the serum resistance associated (SRA) protein. Ann. Soc. Belg Med. Trop. 72(Suppl. 1):13\u201321.","journal-title":"Ann. Soc. Belg Med. 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