{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:25:45Z","timestamp":1790364345981,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cryptococcosis, caused by the basidiomycetous fungus\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            , is responsible for more than 600,000 deaths annually in AIDS patients. Flucytosine is one of the most commonly used antifungal drugs for its treatment, but its resistance and regulatory mechanisms have never been investigated at the genome scale in\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            . In the present study, we performed comparative transcriptome analysis by employing two-component system mutants (\n            <jats:italic>tco1<\/jats:italic>\n            \u0394 and\n            <jats:italic>tco2<\/jats:italic>\n            \u0394) exhibiting opposing flucytosine susceptibility. As a result, a total of 177 flucytosine-responsive genes were identified, and many of them were found to be regulated by Tco1 or Tco2. Among these, we discovered an APSES-like transcription factor, Mbs1 (\n            <jats:underline>Mb<\/jats:underline>\n            p1- and\n            <jats:underline>S<\/jats:underline>\n            wi4-like protein 1). Expression analysis revealed that\n            <jats:italic>MBS1<\/jats:italic>\n            was regulated in response to flucytosine in a Tco2\/Hog1-dependent manner. Supporting this,\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            with the deletion of\n            <jats:italic>MBS1<\/jats:italic>\n            exhibited increased susceptibility to flucytosine. Intriguingly, Mbs1 played pleiotropic roles in diverse cellular processes of\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            . Mbs1 positively regulated ergosterol biosynthesis and thereby affected polyene and azole drug susceptibility. Mbs1 was also involved in genotoxic and oxidative stress responses. Furthermore, Mbs1 promoted production of melanin and capsule and thereby was required for full virulence of\n            <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n            . In conclusion, Mbs1 is considered to be a novel antifungal therapeutic target for treatment of cryptococcosis.\n          <\/jats:p>","DOI":"10.1128\/ec.05236-11","type":"journal-article","created":{"date-parts":[[2011,11,12]],"date-time":"2011-11-12T04:13:09Z","timestamp":1321071189000},"page":"53-67","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["A Flucytosine-Responsive Mbp1\/Swi4-Like Protein, Mbs1, Plays Pleiotropic Roles in Antifungal Drug Resistance, Stress Response, and Virulence of Cryptococcus neoformans"],"prefix":"10.1128","volume":"11","author":[{"given":"Min-Hee","family":"Song","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang-Won","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Su","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ja-Kyung","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore C.","family":"White","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics Division, School of Biological Sciences, University of Missouri, Kansas City, Kansas City, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Floyd","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Heitman","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology, Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K.","family":"Strain","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith N.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Division of Laboratory Animal Medicine, University of North Carolina, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Sun","family":"Bahn","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.23.3206"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/144.3.991"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565110-00002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920009788898019"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1476-1491.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-11-0987"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-02-0113"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.044560"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2165\/00023210-200721040-00004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3601-3609.1999"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.3.6.649"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.10.3389"},{"key":"e_1_3_3_14_2","first-page":"9","article-title":"Mammalian model hosts of cryptococcal infection","volume":"57","author":"Carroll SF","year":"2007","unstructured":"CarrollSF GuillotL QureshiST. 2007. 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