{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:23:38Z","timestamp":1758925418792},"reference-count":45,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We characterized the electrical phenotypes of mutants with mutations in genes encoding calcium transporters\u2014a mechanosensitive channel homolog (\n            <jats:italic>MscS<\/jats:italic>\n            ), a Ca\n            <jats:sup>2+<\/jats:sup>\n            \/H\n            <jats:sup>+<\/jats:sup>\n            exchange protein (\n            <jats:italic>cax<\/jats:italic>\n            ), and Ca\n            <jats:sup>2+<\/jats:sup>\n            -ATPases (\n            <jats:italic>nca-1<\/jats:italic>\n            ,\n            <jats:italic>nca-2<\/jats:italic>\n            ,\n            <jats:italic>nca-3<\/jats:italic>\n            )\u2014as well as those of double mutants (the\n            <jats:italic>nca-2 cax<\/jats:italic>\n            ,\n            <jats:italic>nca-2 nca-3<\/jats:italic>\n            , and\n            <jats:italic>nca-3 cax<\/jats:italic>\n            mutants). The electrical characterization used dual impalements to obtain cable-corrected current-voltage measurements. Only two types of mutants (the\n            <jats:italic>MscS<\/jats:italic>\n            mutant; the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant and\n            <jats:italic>nca-2<\/jats:italic>\n            -containing double mutants) exhibited lower resting potentials. For the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant, on the basis of unchanged conductance and cyanide-induced depolarization of the potential, the cause is attenuated H\n            <jats:sup>+<\/jats:sup>\n            -ATPase activity. The growth of the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant-containing strains was inhibited by elevated extracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            levels, indicative of lesions in Ca\n            <jats:sup>2+<\/jats:sup>\n            homeostasis. However, the net Ca\n            <jats:sup>2+<\/jats:sup>\n            effluxes of the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant, measured noninvasively with a self-referencing Ca\n            <jats:sup>2+<\/jats:sup>\n            -selective microelectrode, were similar to those of the wild type. All of the mutants exhibited osmosensitivity similar to that of the wild type (the turgor of the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant was also similar to that of the wild type), suggesting that Ca\n            <jats:sup>2+<\/jats:sup>\n            signaling does not play a role in osmoregulation. The hyphal tip morphology and tip-localized mitochondria of the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant were similar to those of the wild type, even when the external [Ca\n            <jats:sup>2+<\/jats:sup>\n            ] was elevated. Thus, although Ca\n            <jats:sup>2+<\/jats:sup>\n            homeostasis is perturbed in the\n            <jats:italic>nca-2<\/jats:italic>\n            mutant (B. J. Bowman et al., Eukaryot. Cell 10:654\u2013661, 2011), the phenotype does not extend to tip growth or to osmoregulation but is revealed by lower H\n            <jats:sup>+<\/jats:sup>\n            -ATPase activity.\n          <\/jats:p>","DOI":"10.1128\/ec.05329-11","type":"journal-article","created":{"date-parts":[[2012,3,10]],"date-time":"2012-03-10T04:57:41Z","timestamp":1331355461000},"page":"694-702","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Electrical Phenotypes of Calcium Transport Mutant Strains of a Filamentous Fungus, Neurospora crassa"],"prefix":"10.1128","volume":"11","author":[{"given":"Ahmed","family":"Hamam","sequence":"first","affiliation":[{"name":"York University, Biology Department, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger R.","family":"Lew","sequence":"additional","affiliation":[{"name":"York University, Biology Department, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2008.07.019"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35036035"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5767-5774.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.1.1-108.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00174-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00239-10"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032427999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00235-10"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601456103"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01422"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01554"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2004.07.007"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869898"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.04.039"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1998.1037"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072574"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","unstructured":"KunkelJG\nCordeiroS\nXuY\nShipleyAM\nFeijoJA. 2006. 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