{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:16:31Z","timestamp":1790856991132,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2003,2,1]],"date-time":"2003-02-01T00:00:00Z","timestamp":1044057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            , a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            . We obtained 13,490 expressed sequence tags from both 5\u2032 and 3\u2032 ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several\n            <jats:italic>Candida albicans<\/jats:italic>\n            virulence and pathogenicity homologues in\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            . Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. The first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            , and provide a broader basis for further progress in understanding its biological peculiarities.\n          <\/jats:p>","DOI":"10.1128\/ec.2.1.34-48.2003","type":"journal-article","created":{"date-parts":[[2003,2,11]],"date-time":"2003-02-11T23:36:19Z","timestamp":1045006579000},"page":"34-48","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":145,"title":["Expressed Sequence Tag Analysis of the Human Pathogen\n            <i>Paracoccidioides brasiliensis<\/i>\n            Yeast Phase: Identification of Putative Homologues of\n            <i>Candida albicans<\/i>\n            Virulence and Pathogenicity Genes"],"prefix":"10.1128","volume":"2","author":[{"given":"Gustavo H.","family":"Goldman","sequence":"first","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everaldo","family":"dos Reis Marques","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dio\u0301genes Custo\u0301dio","family":"Duarte Ribeiro","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano A\u0302ngelo","family":"de Souza Bernardes","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301a Carla","family":"Quiapin","sequence":"additional","affiliation":[{"name":"Faculdade de Filosofia, Cie\u0302ncias, e Letras de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patri\u0301cia Marostica","family":"Vitorelli","sequence":"additional","affiliation":[{"name":"Faculdade de Filosofia, Cie\u0302ncias, e Letras de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Savoldi","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camile P.","family":"Semighini","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina C.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Nu\u0301cleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Nunes","sequence":"additional","affiliation":[{"name":"Nu\u0301cleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia, e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"Puccia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia, e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner L.","family":"Batista","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia, e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie Ecker","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Parai\u0301ba, UNIVAP, Vale do Parai\u0301ba, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u0301lio C.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Parai\u0301ba, UNIVAP, Vale do Parai\u0301ba, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Bogossian","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Parai\u0301ba, UNIVAP, Vale do Parai\u0301ba, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredj","family":"Tekaia","sequence":"additional","affiliation":[{"name":"Unite\u0301 de Ge\u0301ne\u0301tique Mole\u0301culaire des Levures, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Pasetto","family":"Nobrega","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Parai\u0301ba, UNIVAP, Vale do Parai\u0301ba, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco G.","family":"Nobrega","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Parai\u0301ba, UNIVAP, Vale do Parai\u0301ba, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Helena S.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Filosofia, Cie\u0302ncias, e Letras de Ribeira\u0303o Preto, Universidade de Sa\u0303o 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