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To gain insight into the adaptation of the fungus to the host central nervous system (CNS), serial analysis of gene expression (SAGE) was used to characterize the gene expression profile of\n            <jats:italic>C. neoformans<\/jats:italic>\n            cells recovered from the CNS of infected rabbits. A SAGE library was constructed, and 49,048 tags were sequenced; 16,207 of these tags were found to represent unique sequences or tag families. Of the 304 most-abundant tags, 164 were assigned to a putative gene for subsequent functional grouping. The results (as determined according to the number of tags that identified genes encoding proteins required for these functions) indicated that the\n            <jats:italic>C. neoformans<\/jats:italic>\n            cells were actively engaged in protein synthesis, protein degradation, stress response, small-molecule transport, and signaling. In addition, a high level of energy requirement of the fungal cells was suggested by a large number of tags that matched putative genes for energy production. Taken together, these findings provide the first insight into the transcriptional adaptation of\n            <jats:italic>C. neoformans<\/jats:italic>\n            to the host environment and identify the set of fungal genes most highly expressed during cerebrospinal fluid infection.\n          <\/jats:p>","DOI":"10.1128\/ec.2.6.1336-1349.2003","type":"journal-article","created":{"date-parts":[[2003,12,10]],"date-time":"2003-12-10T02:17:48Z","timestamp":1071022668000},"page":"1336-1349","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":123,"title":["<i>Cryptococcus neoformans<\/i>\n            Gene Expression during Experimental Cryptococcal Meningitis"],"prefix":"10.1128","volume":"2","author":[{"given":"B. 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