{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:58:47Z","timestamp":1761487127432},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Concanavalin A (ConA) kills the procyclic (insect) form of\n            <jats:italic>Trypanosoma brucei<\/jats:italic>\n            by binding to its major surface glycoprotein, procyclin. We previously isolated a mutant cell line, ConA 1-1, that is less agglutinated and more resistant to ConA killing than are wild-type (WT) cells. Subsequently we found that the ConA resistance phenotype in this mutant is due to the fact that the procyclin either has no N-glycan or has an N-glycan with an altered structure. Here we demonstrate that the alteration in procyclin N-glycosylation correlates with two defects in the N-linked oligosaccharide biosynthetic pathway. First, ConA 1-1 has a defect in activity of polyprenol reductase, an enzyme involved in synthesis of dolichol. Metabolic incorporation of [\n            <jats:sup>3<\/jats:sup>\n            H]mevalonate showed that ConA 1-1 synthesizes equal amounts of dolichol and polyprenol, whereas WT cells make predominantly dolichol. Second, we found that ConA 1-1 synthesizes and accumulates an oligosaccharide lipid (OSL) precursor that is smaller in size than that from WT cells. The glycan of OSL in WT cells is apparently Man\n            <jats:sub>9<\/jats:sub>\n            GlcNAc\n            <jats:sub>2<\/jats:sub>\n            , whereas that from ConA 1-1 is Man\n            <jats:sub>7<\/jats:sub>\n            GlcNAc\n            <jats:sub>2<\/jats:sub>\n            . The smaller OSL glycan in the ConA 1-1 explains how some procyclin polypeptides bear a Man\n            <jats:sub>4<\/jats:sub>\n            GlcNAc\n            <jats:sub>2<\/jats:sub>\n            modified with a terminal\n            <jats:italic>N<\/jats:italic>\n            -acetyllactosamine group, which is poorly recognized by ConA.\n          <\/jats:p>","DOI":"10.1128\/ec.3.2.255-263.2004","type":"journal-article","created":{"date-parts":[[2004,4,9]],"date-time":"2004-04-09T20:03:08Z","timestamp":1081540988000},"page":"255-263","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Defects in the N-Linked Oligosaccharide Biosynthetic Pathway in a\n            <i>Trypanosoma brucei<\/i>\n            Glycosylation Mutant"],"prefix":"10.1128","volume":"3","author":[{"given":"Alvaro","family":"Acosta-Serrano","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, Johns Hopkins School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"O'Rear","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Quellhorst","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Johns Hopkins School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo-Yuan","family":"Hwa","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Johns Hopkins School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon S.","family":"Krag","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul T.","family":"Englund","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Johns Hopkins School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4246"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.42.29763"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1513"},{"key":"e_1_3_2_5_2","first-page":"289","volume":"36","year":"1979","unstructured":"Brun, R., and M. Shonenberger. 1979. Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop.36:289-292.","journal-title":"Acta Trop."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)63815-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950180406"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(86)90083-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2910051"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42769-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07821.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/9.2.181"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00310-8"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0839"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(86)90576-X"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(82)90315-4"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7828"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54990-5"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90684-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/2.4.313"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39781-9"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(98)00168-6"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.180230697"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.6868"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/3.3.193"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.10.6201"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(00)00327-3"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0141"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/9.1.65"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/6.5.493"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90150-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/325272a0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00195-3"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/3.5.481"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77337-7"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.6.1369"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(76)90249-X"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1066"},{"key":"e_1_3_2_39_2","first-page":"229","volume":"3","year":"1996","unstructured":"Welburn, S. C., C. Dale, D. Ellis, R. Beecroft, and T. W. Pearson. 1996. Apoptosis in procyclic Trypanosoma brucei rhodesiense in vitro. Cell Death Differ.3:229-236.","journal-title":"Cell Death Differ."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(82)83008-5"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54916-4"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.3.2.255-263.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.3.2.255-263.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T13:27:14Z","timestamp":1645622834000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.3.2.255-263.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["10.1128\/EC.3.2.255-263.2004"],"URL":"https:\/\/doi.org\/10.1128\/ec.3.2.255-263.2004","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4]]},"assertion":[{"value":"2003-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}