{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:40:04Z","timestamp":1791348004373,"version":"4.3.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The yeast-to-hypha transition is a key feature in the cell biology of the dimorphic human fungal pathogen\n            <jats:italic>Candida albicans<\/jats:italic>\n            . Reorganization of the actin cytoskeleton is required for this dimorphic switch in\n            <jats:italic>Candida<\/jats:italic>\n            . We show that\n            <jats:italic>C. albicans WAL1<\/jats:italic>\n            mutants with both copies of the Wiskott-Aldrich syndrome protein (WASP) homolog deleted do not form hyphae under all inducing conditions tested. Growth of the wild-type and\n            <jats:italic>wal1<\/jats:italic>\n            mutant strains was monitored by in vivo time-lapse microscopy both during yeast-like growth and under hypha-inducing conditions. Isotropic bud growth produced round\n            <jats:italic>wal1<\/jats:italic>\n            cells and unusual mother cell growth. Defects in the organization of the actin cytoskeleton resulted in the random localization of actin patches. Furthermore,\n            <jats:italic>wal1<\/jats:italic>\n            cells exhibited defects in the endocytosis of the lipophilic dye FM4-64, contained increased numbers of vacuoles compared to the wild type, and showed defects in bud site selection. Under hypha-inducing conditions\n            <jats:italic>wal1<\/jats:italic>\n            cells were able to initiate polarized morphogenesis, which, however, resulted in the formation of pseudohyphal cells. Green fluorescent protein (GFP)-tagged Wal1p showed patch-like localization in emerging daughter cells during the yeast growth phase and at the hyphal tips under hypha-inducing conditions. Wal1p-GFP localization largely overlapped with that of actin. Our results demonstrate that Wal1p is required for the organization of the actin cytoskeleton and hyphal morphogenesis in\n            <jats:italic>C. albicans<\/jats:italic>\n            as well as for endocytosis and vacuole morphology.\n          <\/jats:p>","DOI":"10.1128\/ec.3.2.471-482.2004","type":"journal-article","created":{"date-parts":[[2004,4,9]],"date-time":"2004-04-09T20:03:08Z","timestamp":1081540988000},"page":"471-482","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Polarized Hyphal Growth in\n            <i>Candida albicans<\/i>\n            Requires the Wiskott-Aldrich Syndrome Protein Homolog Wal1p"],"prefix":"10.1128","volume":"3","author":[{"given":"A.","family":"Walther","sequence":"first","affiliation":[{"name":"Junior Research Group: Growth Control of Fungal Pathogens, Hans-Kno\u0308ll Institute for Natural Products Research and Department of Microbiology, Friedrich-Schiller 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