{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:53:38Z","timestamp":1783349618580,"version":"3.54.6"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The regulator of G protein signaling homolog Crg1 was found to be a key regulator of pheromone-responsive mating in the opportunistic human fungal pathogen\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    . A mutation in the\n                    <jats:italic>CRG1<\/jats:italic>\n                    gene has greatly increased virulence in the prevalently distributed\n                    <jats:italic>MAT<\/jats:italic>\n                    \u03b1 strains of the fungus. Mouse survival time was shortened by 40%, and the lethal dosage was 100-fold less than that of wild-type strains. In addition, the increased virulence of\n                    <jats:italic>crg1<\/jats:italic>\n                    mutant strains was dependent on the transcription factor homolog Ste12\u03b1 but not on the mitogen-activated protein kinase homolog Cpk1. The enhanced mating due to\n                    <jats:italic>CRG1<\/jats:italic>\n                    mutation, however, was still dependent on Cpk1. Interestingly,\n                    <jats:italic>crg1<\/jats:italic>\n                    mutants of\n                    <jats:italic>MAT<\/jats:italic>\n                    \u03b1 cells produced dark melanin pigment under normally inhibitory conditions, which may relate to the mechanism for increased virulence.\n                  <\/jats:p>","DOI":"10.1128\/ec.3.4.1028-1035.2004","type":"journal-article","created":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T16:54:45Z","timestamp":1092070485000},"page":"1028-1035","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Mutation of the Regulator of G Protein Signaling Crg1 Increases Virulence in\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1128","volume":"3","author":[{"given":"Ping","family":"Wang","sequence":"first","affiliation":[{"name":"Departments of Pediatrics"},{"name":"Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center"},{"name":"Research Institute for Children, New Orleans, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Cutler","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics"},{"name":"Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center"},{"name":"Research Institute for Children, New Orleans, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"King","sequence":"additional","affiliation":[{"name":"Research Institute for Children, New Orleans, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Palmer","sequence":"additional","affiliation":[{"name":"Research Institute for Children, New Orleans, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.23.3206"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt743"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9729965"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.3.1269"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.2.1.11-20.1982"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.2.1.21-29.1982"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02236.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.443-448.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1057740"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03563.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(99)01515-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.235"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.7.12.4169-4177.1987"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.9.3179-3191.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00007-6"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/156.4.1585"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.5.1036-1045.2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(99)01369-3"},{"key":"e_1_3_2_21_2","first-page":"428","volume":"94","year":"1996","unstructured":"Hepler, J. R., M. D. Berman, A. G. Gilman, and T. Kozasa. 1996. RGS4 and GAIP are GTPase-activating proteins for Gq\u03b1 and block activation of phospholipase C\u03b2 by \u03b3-thio-GTP-Gq\u03b1. Proc. Natl. Acad. Sci. USA94:428-432.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.21.7102-7104.1993"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(20000330)16:5<387::AID-YEA525>3.0.CO;2-U"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.2.546-552.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80055-5"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.4049\/jimmunol.129.4.1675","volume":"129","year":"1982","unstructured":"Kozel, T. R., and E. C. Gotschlich. 1982. The capsule of Cryptococcus neoformans passively inhibits phagocytosis of the yeast by macrophages. J. Immunol.129:1675-1680.","journal-title":"J. Immunol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/10.Supplement_2.S436"},{"key":"e_1_3_2_28_2","unstructured":"Kwon-Chung K. J. and J. E. Bennett. 1992. Cryptococcosis p. 397-446. In Medical mycology. Lea & Febiger Malvern Pa."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.4.746-785.2000"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14455"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091096998"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.515"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.13.3.1962-1970.1993"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4831-4841.2003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb941"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1405-1411.1993"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.39.5.383.386"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve109"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.2.257-272.2002"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.1.352-362.2000"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.3131-3136.1995"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.7.3004-3007.1994"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437995100"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00903.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/153.4.1601"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1251\/bpo68"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.3.4.1028-1035.2004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.3.4.1028-1035.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:48:04Z","timestamp":1682729284000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.3.4.1028-1035.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,8]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1128\/EC.3.4.1028-1035.2004"],"URL":"https:\/\/doi.org\/10.1128\/ec.3.4.1028-1035.2004","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1023154.264841","asserted-by":"object"}]},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,8]]},"assertion":[{"value":"2004-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-06-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}