{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:48:39Z","timestamp":1790498919968,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    is an opportunistic fungal pathogen whose virulence is related to its ability to switch between yeast, pseudohyphal, and true-hyphal morphologies. To ask how long-distance nuclear migration occurs in\n                    <jats:italic>C. albicans<\/jats:italic>\n                    hyphae, we identified the fundamental properties of nuclear movements and microtubule dynamics using time-lapse microscopy. In hyphae, nuclei migrate to, and divide across, the presumptive site of septation, which forms 10 to 15 \u03bcm distal to the basal cell. The mother nucleus returns to the basal cell, while the daughter nucleus reiterates the process. We used time-lapse microscopy to identify the mechanisms by which\n                    <jats:italic>C. albicans<\/jats:italic>\n                    nuclei move over long distances and are coordinated with hyphal morphology. We followed nuclear migration and spindle dynamics, as well as the time and position of septum specification, defined it as the presumptum, and established a chronology of nuclear, spindle, and morphological events. Analysis of microtubule dynamics revealed that premitotic forward nuclear migration is due to the repetitive sliding of astral microtubules along the cell cortex but that postmitotic forward and reverse nuclear migrations are due primarily to spindle elongation. Free microtubules exhibit cell cycle regulation; they are present during interphase and disappear at the time of spindle assembly. Finally, a growth defect in strains expressing Tub2-green fluorescent protein revealed a connection between hyphal elongation and the nuclear cell cycle that is coordinated by hyphal length and\/or volume.\n                  <\/jats:p>","DOI":"10.1128\/ec.4.10.1697-1711.2005","type":"journal-article","created":{"date-parts":[[2005,10,7]],"date-time":"2005-10-07T13:57:57Z","timestamp":1128693477000},"page":"1697-1711","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Microtubules in\n                    <i>Candida albicans<\/i>\n                    Hyphae Drive Nuclear Dynamics and Connect Cell Cycle Progression to Morphogenesis"],"prefix":"10.1128","volume":"4","author":[{"given":"Kenneth R.","family":"Finley","sequence":"first","affiliation":[{"name":"Departments of Genetics, Cell Biology, and Development"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Berman","sequence":"additional","affiliation":[{"name":"Departments of Genetics, Cell Biology, and Development"},{"name":"Microbiology, University of Minnesota, Minneapolis, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.4.863"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-2-271"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90418-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.124.1.511-523.1975"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.3.629"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02414"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00530-X"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00413-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.10.5953-5959.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00352202"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.6.1321"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.11.3538"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.738"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00338-7"},{"key":"e_1_3_2_16_2","first-page":"2195","volume":"128","year":"1982","unstructured":"Gow, N. A., and G. W. Gooday. 1982. Vacuolation, branch production and linear growth of germ tubes in Candida albicans. J. Gen. Microbiol.128:2195-2198.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_17_2","first-page":"97","volume":"47","year":"1986","unstructured":"Gow, N. A., G. Henderson, and G. W. Gooday. 1986. Cytological interrelationships between the cell cycle and duplication cycle of Candida albicans. Microbios47:97-105.","journal-title":"Microbios"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970200106"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.89.3.343"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-03-0116"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6611"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-09-0798"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01650"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200302030"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-03-0254"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.07.012"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.222602"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00119-3"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80358-X"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1574-1588.2004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.569"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.6.1255"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-003-0020-8"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.4.971"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.5.1053-1060.2003"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.4.985"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)94004-V"},{"key":"e_1_3_2_38_2","first-page":"167","year":"1985","unstructured":"Soll, D. R. 1985. Candida albicans, p. 167-195. In P. J. Szaniszlo (ed.), Fungal dimorphism: with emphasis on fungi pathogenic to humans. Plenum Publishing Co., New York, N.Y.","journal-title":"Fungal dimorphism: with emphasis on fungi pathogenic to humans"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90356-6"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(78)90077-0"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.3.609"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-08-0513"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.05.008"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02459.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00415-5"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.5.993"},{"key":"e_1_3_2_47_2","first-page":"496","volume":"3","year":"2004","unstructured":"Torres-Rosell, J., F. Machin, A. Jarmuz, and L. Aragon. 2004. Nucleolar segregation lags behind the rest of the genome and requires Cdc14p activation by the FEAR network. Cell Cycle.3:496-502.","journal-title":"Cell Cycle."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.3.7.1003"},{"key":"e_1_3_2_49_2","first-page":"2732\u01102746","volume":"13","year":"2002","unstructured":"Warenda, A. J., and J. B. Konopka. 2002. Septin function in Candida albicans morphogenesis. Mol. Biol. Cell13:2732\u01102746.","journal-title":"Mol. Biol. Cell"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00515-5"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.6.1868-1874.1999"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00291048"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-6-1067"}],"container-title":["Eukaryotic Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.4.10.1697-1711.2005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/EC.4.10.1697-1711.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T08:32:44Z","timestamp":1645605164000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/EC.4.10.1697-1711.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10]]},"references-count":52,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1128\/EC.4.10.1697-1711.2005"],"URL":"https:\/\/doi.org\/10.1128\/ec.4.10.1697-1711.2005","relation":{},"ISSN":["1535-9778","1535-9786"],"issn-type":[{"value":"1535-9778","type":"print"},{"value":"1535-9786","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10]]},"assertion":[{"value":"2005-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-07-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}