{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:16:31Z","timestamp":1790856991122,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            is a thermodimorphic fungus associated with paracoccidioidomycosis (PCM), a systemic mycosis prevalent in South America. In humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and transform into the yeast parasitic form. Thus, the mycelium-to-yeast transition is of particular interest because conversion to yeast is essential for infection. We have used a\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            biochip carrying sequences of 4,692 genes from this fungus to monitor gene expression at several time points of the mycelium-to-yeast morphological shift (from 5 to 120 h). The results revealed a total of 2,583 genes that displayed statistically significant modulation in at least one experimental time point. Among the identified gene homologues, some encoded enzymes involved in amino acid catabolism, signal transduction, protein synthesis, cell wall metabolism, genome structure, oxidative stress response, growth control, and development. The expression pattern of 20 genes was independently verified by real-time reverse transcription-PCR, revealing a high degree of correlation between the data obtained with the two methodologies. One gene, encoding 4-hydroxyl-phenyl pyruvate dioxygenase (4-HPPD), was highly overexpressed during the mycelium-to-yeast differentiation, and the use of NTBC [2-(2-nitro-4-trifluoromethylbenzoyl)-cyclohexane-1,3-dione], a specific inhibitor of 4-HPPD activity, as well as that of NTBC derivatives, was able to inhibit growth and differentiation of the pathogenic yeast phase of the fungus in vitro. These data set the stage for further studies involving NTBC and its derivatives as new chemotherapeutic agents against PCM and confirm the potential of array-based approaches to identify new targets for the development of alternative treatments against pathogenic microorganisms.\n          <\/jats:p>","DOI":"10.1128\/ec.4.12.2115-2128.2005","type":"journal-article","created":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T23:23:25Z","timestamp":1134084205000},"page":"2115-2128","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Transcriptome Analysis of\n            <i>Paracoccidioides brasiliensis<\/i>\n            Cells Undergoing Mycelium-to-Yeast Transition"],"prefix":"10.1128","volume":"4","author":[{"given":"Luiz R.","family":"Nunes","sequence":"first","affiliation":[{"name":"Nu\u0301cleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, 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Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0301rcia Eliana","family":"da Silva Ferreira","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dio\u0301genes Custo\u0301dio Duarte","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Paraiba, Sao Jose dos Campos, SP, 12244-000, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano A\u0302ngelo","family":"de Souza Bernardes","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Helena S.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Filosofia, Cie\u0302ncias e Letras de Ribeira\u0303o Preto, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"Puccia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner L.","family":"Batista","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Pasetto","family":"No\u0301brega","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Paraiba, Sao Jose dos Campos, SP, 12244-000, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco G.","family":"Nobrega","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Paraiba, Sao Jose dos Campos, SP, 12244-000, Sa\u0303o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding-Yah","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tunghai University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"de Braganc\u0327a Pereira","sequence":"additional","affiliation":[{"name":"Instituto de Matema\u0301tica e Estati\u0301stica, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, 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