{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:16:37Z","timestamp":1791170197185,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Eukaryot Cell"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to demonstrate that a chromosomal translocation and segmental duplication occurred during construction of a congenic strain pair in the fungal human pathogen\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            . Two chromosomes underwent telomere-telomere fusion, generating a dicentric chromosome that broke to produce a chromosomal translocation, forming two novel chromosomes sharing a large segmental duplication. The duplication spans 62,872 identical nucleotides and generated a second copy of 22 predicted genes, and we hypothesize that this event may have occurred during meiosis. Gene disruption studies of one embedded gene (\n            <jats:italic>SMG1<\/jats:italic>\n            ) corroborate that this region is duplicated in an otherwise haploid genome. These findings resolve a genome project assembly anomaly and illustrate an example of rapid genome evolution in a fungal genome rich in repetitive elements.\n          <\/jats:p>","DOI":"10.1128\/ec.4.2.401-406.2005","type":"journal-article","created":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T20:18:15Z","timestamp":1107893895000},"page":"401-406","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["Chromosomal Translocation and Segmental Duplication in\n            <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1128","volume":"4","author":[{"given":"James A.","family":"Fraser","sequence":"first","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"},{"name":"Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnny C.","family":"Huang","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Read","family":"Pukkila-Worley","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"},{"name":"Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Andrew","family":"Alspaugh","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"},{"name":"Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Heitman","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics and Microbiology"},{"name":"Medicine"},{"name":"Pharmacology and Cancer Biology and"},{"name":"Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.GR-1871R"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002940050267"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.148600"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-8-2607"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0904-932"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095781"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02579"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242624799"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.12.6.2777-2783.1992"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.337602"},{"key":"e_1_3_2_12_2","first-page":"937","volume":"2","year":"2004","unstructured":"Fortna, A., Y. 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